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Development and Application of In Vitro Culture Techniques in Plants—2nd...
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Development and Application of In Vitro Culture Techniques in Plants—2nd Edition

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Cell Biology".

Deadline for manuscript submissions: 31 January 2027 | Viewed by 1566

Editors

Dr. Ramon Dolcet-Sanjuan

E-Mail Website
Guest Editor
IRTA, Fruitcentre, Plant In Vitro Culture Laboratory, Fruticulture Program, Parc AgroBiotech, 25003 Lleida, Spain
Interests: plant in vitro culture to horticultural breeding; propagation; doubled haploid lines; cell suspensions; fruit and forest trees; vegetables; ornamentals
Special Issues, Collections and Topics in MDPI journals
Dr. Jean Carlos Cardoso

E-Mail Website
Guest Editor
Department of Biotechnology, Plant and Animal Production, Centro de Ciências Agrárias, Universidade Federal de São Carlos, Araras, SP 13600-970, Brazil
Interests: floriculture; breeding; propagation; in vitro plant cultivation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The techniques developed within plant in vitro culture are diverse and depend on the purposes or objectives of each research, development and innovation laboratory, mainly determined by the plant species they are working on or commercializing. This Special Issue gathers general information, illustrated with examples, with the purpose of orienting future research and development groups. This issue will compile methodologies for (1) micropropagation, (2) development and application of bioreactors, (2) sanitation or virus elimination, (3) adventitious regeneration, (4) somatic embryogenesis, (5) protoplast culture and somatic hybrids, (6) production of doubled haploid plants by androgenesis, gynogenesis, or parthenogenesis, (7) rescue of immature embryos or interspecific crosses, (8) cell suspensions as a source of metabolites and food, (9) bioassays for biotic and abiotic stress agents, (10) protocol improvement aimed at genetic transformation or for editing tools, (11) control of endophytes under in vitro culture and (12) beneficial effects of microorganisms in plant in vitro culture.

Dr. Ramon Dolcet-Sanjuan
Dr. Jean Carlos Cardoso
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • micropropagation
  • bioreactors
  • sanitation
  • adventitious regeneration
  • somatic embryogenesis
  • protoplast
  • doubled haploids
  • rescue of immature embryos
  • cell suspensions
  • bioassays

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Related Special Issue

Published Papers (2 papers)

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Research

27 pages, 20598 KB  
Article
Multiple Shoot Bud Induction and Plant Regeneration in Madhuca indica J.F.Gmel.: Histological, Genetic Fidelity and GC-MS Analysis
by Zishan Ahmad, Vikas Yadav, Anwar Shahzad, Anamica Upadhaya and Muthusamy Ramakrishnan
Plants 2026, 15(12), 1921; https://doi.org/10.3390/plants15121921 - 22 Jun 2026
Viewed by 595
Abstract
Madhuca indica J.F.Gmel. holds significant economic and industrial value due to its applications in traditional and modern medicine. Its oil is especially important for biodiesel production, owing to its high acid value and suitability as a non-edible feedstock. However, propagation is difficult due [...] Read more.
Madhuca indica J.F.Gmel. holds significant economic and industrial value due to its applications in traditional and modern medicine. Its oil is especially important for biodiesel production, owing to its high acid value and suitability as a non-edible feedstock. However, propagation is difficult due to low seed germination, seed recalcitrance, and poor rooting of stem cuttings, limiting large-scale multiplication through conventional methods. To address these limitations, a regeneration protocol using nodal explants was developed. Murashige and Skoog (MS) medium augmented with BA (5.0 µM) and NAA (0.5 µM) produced a maximum of 7.10 ± 0.11 shoots per explant with an average shoot length of 4.53 ± 0.22 cm after six weeks. Rooting was achieved on half-strength medium supplemented with IBA (1.0 µM), resulting in 4.83 ± 0.17 roots per shoot and a root length of 4.50 ± 0.20 cm. In vitro-derived plants were successfully acclimatised in Soilrite with an 82.3% survival rate. The explants were derived from aseptic seedling material, representing juvenile rather than mature elite donor sources. Direct shoot bud development was verified by histological examination. Within the resolution of the employed marker systems, no polymorphism was found utilising RAPD and ISSR markers. SEM showed similar leaf surface characteristics, and physiological and biochemical studies were carried out throughout acclimatisation. A partial overlap in metabolite composition with qualitative and relative quantitative differences between mother and in vitro-derived plants was shown by GC–MS-based profiling. Overall, the study establishes a reproducible regeneration system for M. indica, providing a basis for further optimisation and conservation-oriented applications. Full article
(This article belongs to the Special Issue Development and Application of In Vitro Culture Techniques in Plants—2nd Edition)
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15 pages, 3110 KB  
Article
In Vitro Micropropagation of Rosa canina L.: From Establishment to Plant Acclimatization
by Maria Casanovas, Emma Roca and Ramon Dolcet-Sanjuan
Plants 2026, 15(9), 1285; https://doi.org/10.3390/plants15091285 - 22 Apr 2026
Viewed by 644
Abstract
Rosa canina L. is a medicinal and nutritionally valuable species with increasing industrial demand, yet its conventional propagation is limited by low rooting capacity and high genetic heterogeneity. In this study, a complete and reproducible in vitro micropropagation protocol was established, from explant [...] Read more.
Rosa canina L. is a medicinal and nutritionally valuable species with increasing industrial demand, yet its conventional propagation is limited by low rooting capacity and high genetic heterogeneity. In this study, a complete and reproducible in vitro micropropagation protocol was established, from explant introduction to plantlet acclimatization. Axillary buds were disinfected and introduced into Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP). Shoot multiplication was achieved using sequential cytokinin treatments, and shoot elongation was promoted by adding liquid MS medium containing activated charcoal (AC). The highest and fastest root induction percentage (up to 75%) was obtained on WPM with 2 mg·L−1 IBA and under a 16 h light/8 h dark photoperiod. Light promoted adventitious root formation depending on the nutrient formulation. Thereafter, shoots developed well-structured root systems in vitro, and plantlets fully survived to ex vitro acclimatization. This protocol provides an efficient platform for the large-scale propagation of R. canina and demonstrates that its auxin-driven adventitious rooting is strongly conditioned by the interaction between basal medium composition and photoperiod. Full article
(This article belongs to the Special Issue Development and Application of In Vitro Culture Techniques in Plants—2nd Edition)
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