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Development and Application of In Vitro Culture Techniques in Plants
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Development and Application of In Vitro Culture Techniques in Plants

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Cell Biology".

Deadline for manuscript submissions: 30 December 2025 | Viewed by 3216

Special Issue Editors

Dr. Ramon Dolcet-Sanjuan

E-Mail Website
Guest Editor
Plant in Vitro Culture Laboratory, Fruticulture Program, IRTA, Fruitcentre, Parc AgroBiotech, 25003 Lleida, Spain
Interests: plant in vitro culture to horticultural breeding; propagation; doubled haploid lines; cell suspensions; fruit and forest trees; vegetables; ornamentals
Dr. Jean Carlos Cardoso

E-Mail Website
Guest Editor
Department of Biotechnology, Plant and Animal Production, Centro de Ciências Agrárias, Universidade Federal de São Carlos, Araras 13600-970, SP, Brazil
Interests: floriculture; breeding; propagation; in vitro plant cultivation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The techniques developed within plant in vitro culture are diverse and depend on the purposes or objectives of each research, development, and innovation laboratory, mainly determined by the plant species they are working on or commercializing. This Special Issue gathers general information, illustrated with examples, with the purpose of orienting future research and development groups. This issue will compile methodologies for (1) micropropagation, (2) development and application of bioreactors, (2) sanitation or virus elimination, (3) adventitious regeneration, (4) somatic embryogenesis, (5) protoplast culture and somatic hybrids, (6) production of doubled haploid plants by androgenesis, gynogenesis, or parthenogenesis, (7) rescue of immature embryos or interspecific crosses, (8) cell suspensions as a source of metabolites and food, (9) bioassays for biotic and abiotic stress agents, (10) protocol improvement aimed at genetic transformation or for editing tools, (11) control of endophytes under in vitro culture, and (12) beneficial effects of microorganisms in plant in vitro culture.

Dr. Ramon Dolcet-Sanjuan
Dr. Jean Carlos Cardoso
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • micropropagation
  • bioreactors
  • sanitation
  • adventitious regeneration
  • somatic embryogenesis
  • protoplast
  • doubled haploids
  • rescue of immature embryos
  • cell suspensions
  • bioassays

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Published Papers (3 papers)

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Research

26 pages, 28140 KiB  
Article
Response to In Vitro Micropropagation of Plants with Different Degrees of Variegation of the Commercial Gymnocalycium cv. Fancy (Cactaceae)
by Carles Cortés-Olmos, Vladimir Marín Guerra-Sandoval, Carla Guijarro-Real, Benito Pineda, Ana Fita and Adrián Rodríguez-Burruezo
Plants 2025, 14(7), 1091; https://doi.org/10.3390/plants14071091 - 1 Apr 2025
Viewed by 365
Abstract
This study aims to establish efficient in vitro propagation protocols for Gymnocalycium cv. Fancy, an ornamental cactus with variegated variants, by evaluating the effects of cytokinin type and explant source on the organogenic response. Plants with different degrees of variegation (0–100%) were classified [...] Read more.
This study aims to establish efficient in vitro propagation protocols for Gymnocalycium cv. Fancy, an ornamental cactus with variegated variants, by evaluating the effects of cytokinin type and explant source on the organogenic response. Plants with different degrees of variegation (0–100%) were classified by size to obtain different explant types (apices, central discs, epicotyls, and hypocotyls). The effects of 6-Benzylaminopurine (BAP, 8 µM), Kinetin (KIN, 4 µM), and Thidiazuron (TDZ, 1 µM) were assessed on shoot production, callus formation, and rhizogenesis. Additionally, we studied the relationship between initial plant variegation and the productivity of the variegated shoots. The best shoot production results were obtained for central discs treated with 1 µM TDZ. Furthermore, a correlation was observed between the activated areole type (green, mixed, or fully colored) and shoot color percentage, enabling precise explant selection. The appearance of differently colored shoots confirms the potential for selecting new lines from this cultivar too. These findings hold significant potential not only for the breeding and propagation of ornamental cacti but also for the cultivation of other edible cacti and their relatives. Full article
(This article belongs to the Special Issue Development and Application of In Vitro Culture Techniques in Plants)
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19 pages, 5097 KiB  
Article
Development of a Feasible and Efficient In Vitro Rescue Protocol for Immature Prunus spp. Embryos
by Maria Casanovas, Elisabet Claveria and Ramon Dolcet-Sanjuan
Plants 2024, 13(21), 2953; https://doi.org/10.3390/plants13212953 - 22 Oct 2024
Viewed by 976
Abstract
The major factors affecting the in vitro immature embryo rescue efficiencies from Prunus persica or P. armeniaca accessions have been identified, along with improving the feasibility. Variations in the woody plant medium (WPM) were used depending on the embryo size. Embryos less than [...] Read more.
The major factors affecting the in vitro immature embryo rescue efficiencies from Prunus persica or P. armeniaca accessions have been identified, along with improving the feasibility. Variations in the woody plant medium (WPM) were used depending on the embryo size. Embryos less than 5 mm long were cultured in WPM supplemented with 1 μM BAP and 1 μM GA3, while embryos bigger than 5 mm long were cultured in hormone-free medium, with or without vermiculite. The environmental in vitro culture conditions consisted of three phases: a (I) stratification at 4 °C during a 3- to 5-month-long period in the dark, followed by (II) growth of germinated embryos at 14 °C for a 4-week-long period, with 12 h light a day, which favors plantlet development, and finally, (III) growth at 24 °C, with 16 h light a day, until the plantlets were acclimatized in the greenhouse. The germination of smaller embryos, at the end of phase I, ranged from 82.2% to 22.1% for apricots and flat peaches, respectively, whereas for bigger embryos, the germination varied from 97.3% to 53.2% for the same species. The embryo germination for peaches and nectarines ranged from 40.1% to 30.3% for smaller embryos, and from 91.9% to 63.0% for bigger embryos. Endo- and epiphytic contamination, affecting from 7.4% to 52.9% of cultured embryos, depending on the fruit type and conservation conditions, and the capacity to acclimate to soil conditions, ranging from 50.4% to 93.2%, were the two most important factors influencing the protocol’s efficiency and feasibility. Considering the overall efficiencies, expressed as hardened plants transferred to field plots over clean uncontaminated embryo, the values ranged from 55.8% for nectarines, 54.0% for peaches, 45.6% for apricots, and 23.3% for flat fruits. The addition of vermiculite to the culture medium significantly improved the plantlet development, avoiding subculture to fresh medium when an extension of phase III was required before acclimatization. Compared to laboratory glassware, the use of food glass containers with air-permeable sealing film, along with vermiculite-containing medium, significantly reduced the costs when handling the large number of embryos required for breeding programs. Full article
(This article belongs to the Special Issue Development and Application of In Vitro Culture Techniques in Plants)
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15 pages, 1906 KiB  
Article
Development, Chlorophyll Content, and Nutrient Accumulation in In Vitro Shoots of Melaleuca alternifolia under Light Wavelengths and 6-BAP
by Antony Cristhian Gonzales-Alvarado and Jean Carlos Cardoso
Plants 2024, 13(20), 2842; https://doi.org/10.3390/plants13202842 - 11 Oct 2024
Cited by 2 | Viewed by 1350
Abstract
In vitro cultivation of Melaleuca could contribute to the cloning of superior genotypes. Studies of factors affecting micropropagation are needed, such as the interaction with light-emitting diodes (LEDs) and plant growth regulators added to the culture media. This study aimed at better understanding [...] Read more.
In vitro cultivation of Melaleuca could contribute to the cloning of superior genotypes. Studies of factors affecting micropropagation are needed, such as the interaction with light-emitting diodes (LEDs) and plant growth regulators added to the culture media. This study aimed at better understanding the effects of spectra on the development and physiology of melaleuca cultivated in vitro, as well as the interaction of LEDs with the main cytokinin used in micropropagation, N6-Benzylaminopurine (6-BAP). 6-BAP, spectra, and their interaction had a significant effect on most of the variables analyzed, altering the in vitro development and chlorophyll concentrations in the plants, as well as changing different variables in the culture medium, such as pH, EC, and levels of Ca2+, Mg2+, and P, and nutrient accumulation in the shoots. The results demonstrate that the main effects of adding BAP to the in vitro cultivation of melaleuca are an increase in the number of shoots, which resulted in greater fresh and dry masses; a reduction in height and chlorophyll content; complete inhibition of adventitious rooting; higher consumption of Mg, and lower consumption of Ca and P from the culture medium; higher content of Fe, and lower content of P, S, Mn, Cu and B in the in vitro shoot tissues. Full article
(This article belongs to the Special Issue Development and Application of In Vitro Culture Techniques in Plants)
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