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Superresolution Optical Microscopy
Special Issue Information
Dear Colleagues,
Presently, a variety of methods exist that overcome the hundred-year-old theoretical limit for the resolution potential of light microscopy (of about 200 nm for visible light), which for many decades has precluded a direct glimpse of the molecular machinery of life. In this Special Issue, we plan to summarize the state of the art from the methodological point of view, and to address some of the challenges still to be resolved before the SRM approaches will be fit to bring about the revolution envisaged in biology, medicine, and the material sciences. In this context, contributions to these subjects are welcome to include optical, physico-chemical, technical and image processing topics.
Prof. Dr. Christoph Cremer
Prof. Dr. Rainer Heintzmann
Prof. Dr. Jennifer Lippincott-Schwartz
Guest Editor
Manuscript Submission Information
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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Photonics is an international peer-reviewed open access monthly journal published by MDPI.
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Keywords
- Methods of SRM (Superresolution microscopy), e.g. focused nanoscopy like 4Pi, STED, RESOLFT
- localization microscopy
- structured illumination
- expansion microscopy
- Resolution issues, e.g. localization precision & accuracy, labelling efficiency, optical resolution, structural resolution, emitter density, Nyquist and Fourier ring correlated criteria
- Photophysics of SRM, e.g. light induced photoconversation, “bright-dark” and “dark-bright” transitions
- use of photostable emitters
- Labelling techniques, e.g. fluorescent proteins, standard dyes, special dyes
- Detection technology, image processing, deconvolution, data mining, data storage
- Applications of SRM (e.g. membranes, nuclear nanostructure, bacteria, viruses
- material sciences)
- Perspectives of SRM (e.g. in terms of instrumentation, resolution, in vivo, high throughput, working distance, field of view)
- Sample preparation techniques and protocols specifically relevant to SRM.
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Published Papers
Photonics - ISSN 2304-6732