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Pathogens
Special Issues
Reverse Microbial Etiology in Plants
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Reverse Microbial Etiology in Plants

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: closed (31 July 2022) | Viewed by 6521

Special Issue Editors

Prof. Dr. Jian Ye

E-Mail Website
Guest Editor
Department of Agricultural Microbiology and Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Interests: arboviruses; vector-borne diseases; plant immunity; pathogen-insect-plant tripartite interactions
Prof. Dr. Renyi Liu

E-Mail Website
Guest Editor
Center for Agroforestry Mega Data Science, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China
Interests: plant viruses; bioinformatics; plant genomics; plant metabolomics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Emerging infectious diseases (EID) caused by unknown microbes poses significant threats to global health, global crop yield, and global security, exemplified by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and whitefly-transmitted geminivirus. Koch’s postulates are at the heart of traditional microbial etiology only when the microbial etiology of an outbreak. After then we could develop strategies such as resistance cultivars to prevent next disease occurrence. However, we know very little about the microbial world on Earth in terms of taxonomy and their traits that can cause a disease. If the potential of EID caused by unknown microbes could be predicated, it should be preventable and results in much less loss. It is necessary to systematically carry out unknown microbial discovery and reverse pathogenic etiology research in a prospective manner, and actively defend against emerging infectious diseases in the future.

  “Reverse microbial etiology” is a new research area aimed to prospective prediction, prevention and control of future infectious disease challenges. It is an indispensable strategy for early warning and response to emerging infectious diseases in the future. Microbial pathogens heavily reduce the crop yield and quality annually, yet known pathogens are only a tip of the iceberg. A large number of potential plant pathogens transmitted by insect vector or stored in the environment have not been discovered. The new emerging crop diseases in the future will be caused by new potential pathogens, which may be identified by metagenomics-based field survey and investigated in advance.  The theme of this special Issue is “Reverse microbial etiology in plants”, which focuses on discovering and isolating new plant viruses, bacteria, fungi or other parasites that may cause disease outbreaks in the future, evaluating the potential pathogenicity, and studying the infection mechanisms, virulence factors, host range, transmission, and evolution. It will lay a foundation for us to explore strategy for detection, prevention and control of crop diseases before the potential outbreak.

For the Special Issue of Pathogens, we invite you to submit research articles, review articles, short notes, as well as communications related to reverse microbial etiology in plant. This Special Issue will provide an overview and list of potential pathogens that may cause new plant diseases, and provide theoretical basis and technical guidance for prevention and control of potential pathogens.

We look forward to your contribution.

Prof. Dr. Jian Ye
Prof. Dr. Renyi Liu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • reverse microbial etiology
  • vector-borne pathogen
  • plant disease
  • metagenomics
  • virulence factor

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Published Papers (2 papers)

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Research

11 pages, 1178 KiB  
Article
Patterns of Genetic Diversity among Alphasatellites Infecting Gossypium Species
by Muhammad Mubin, Arzoo Shabbir, Nazia Nahid, Iram Liaqat, Muhammad Hassan, Nada H. Aljarba, Ahmed Al Qahtani, Claude M. Fauquet, Jian Ye and Muhammad Shah Nawaz-ul-Rehman
Pathogens 2022, 11(7), 763; https://doi.org/10.3390/pathogens11070763 - 4 Jul 2022
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Abstract
Alphasatellites are small single-stranded circular DNA molecules associated with geminiviruses and nanoviruses. In this study, a meta-analysis of known alphasatellites isolated from the genus Gossypium (cotton) over the last two decades was performed. The phylogenetic and pairwise sequence identity analysis suggested that cotton-infecting [...] Read more.
Alphasatellites are small single-stranded circular DNA molecules associated with geminiviruses and nanoviruses. In this study, a meta-analysis of known alphasatellites isolated from the genus Gossypium (cotton) over the last two decades was performed. The phylogenetic and pairwise sequence identity analysis suggested that cotton-infecting begomoviruses were associated with at least 12 different alphasatellites globally. Three out of twelve alphasatellite were associated with cotton leaf curl geminiviruses but were not isolated from cotton plants. The cotton leaf curl Multan alphasatellite, which was initially isolated from cotton, has now been reported in several plant species, including monocot plants such as sugarcane. Our recombination analysis suggested that four alphasatellites, namely cotton leaf curl Lucknow alphasatellites, cotton leaf curl Multan alphasatellites, Ageratum yellow vein Indian alphasatellites and Ageratum enation alphasatellites, evolved through recombination. Additionally, high genetic variability was detected among the cotton-infecting alphasatellites at the genome level. The nucleotide substitution rate for the replication protein of alphasatellites (alpha-Rep) was estimated to be relatively high (~1.56 × 10−3). However, unlike other begomoviruses and satellites, the first codon position of alpha-Rep rapidly changed compared to the second and third codon positions. This study highlights the biodiversity and recombination of alphasatellites associated with the leaf curl diseases of cotton crops. Full article
(This article belongs to the Special Issue Reverse Microbial Etiology in Plants)
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11 pages, 3569 KiB  
Article
Establishment and Application of a Multiplex PCR Assay for the Rapid Detection of Rhizoctonia solani Anastomosis Group (AG)-3PT, the Pathogen Causing Potato Black Scurf and Stem Canker
by Linda Iradukunda, Yan-Ping Wang, Oswald Nkurikiyimfura, Tian Wang, Li-Na Yang and Jiasui Zhan
Pathogens 2022, 11(6), 627; https://doi.org/10.3390/pathogens11060627 - 29 May 2022
Cited by 12 | Viewed by 3073
Abstract
Rhizoctonia solani anastomosis group 3 (AG-3) is the main causative agent of the soil-borne disease known as potato black scurf, which poses a huge threat to potato production. Rapid and accurate identification of R. solani AG-3 isolates in soil and potato seed tubers [...] Read more.
Rhizoctonia solani anastomosis group 3 (AG-3) is the main causative agent of the soil-borne disease known as potato black scurf, which poses a huge threat to potato production. Rapid and accurate identification of R. solani AG-3 isolates in soil and potato seed tubers prior to planting is essential for good production. In this study, a multiplex PCR assay was established for the detection of R. solani AG-3. Two pairs of target-specific primers were designed from sequences for endopolygalacturonase and pyridoxine biosynthesis genes downloaded from GenBank. The main factors influencing PCR amplification, such as annealing temperature and primer concentration, were optimized. Results show that the proposed multiplex PCR assay is highly sensitive and specific for the target genes in the pathogen even when the DNA concentration is reduced to 20 fg/μL. The resulting calibration plot shows a linear relationship between electrophoretic band peaks and genomic DNA concentration (R2 = 0.98). The primer specificity was confirmed by applying them to other R. solani AG groups and plant pathogen species on which no amplicons were produced. Using the primers, we successfully detected small amounts of R. solani AG-3 present in soil and potato tuber samples. Taken together, the detection assay developed in this study has high sensitivity, strong specificity, and accuracy and can be used to detect and identify soil and potato seed tubers infected with Rhizoctonia solani AG-3. Full article
(This article belongs to the Special Issue Reverse Microbial Etiology in Plants)
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