Next-Gen Sequencing Advances Pathogen Research in Health, Agriculture & Environment

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: 28 February 2026 | Viewed by 496

Special Issue Editors


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Guest Editor
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Interests: next generation seq; microbiological identification

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Guest Editor
Instituto de Genética Veterinaria (IGEVET), CONICET, La Plata, Argentina
Interests: genetic; NGS
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Special Issue Information

Dear Colleagues,

This Pathogens Special Issue emphasizes the crucial role of next-generation sequencing (NGS) and advanced molecular techniques in addressing the global health challenges posed by infectious pathogens. These pathogens not only threaten human and animal health but also cause significant economic losses and threaten biodiversity. By using high-throughput sequencing technologies among other molecular techniques, researchers can rapidly identify, characterize, and monitor pathogens, thereby enhancing our molecular and epidemiological understanding. This collection highlights how cutting-edge technology contributes to improvements in disease monitoring, control, and management strategies across public health, agriculture, livestock, and environmental sectors. This issue features case studies demonstrating how advancements in sequencing reveal insights into pathogen epidemiology, phylogeny, the emergence of new strains, and resistance development. Ultimately, this Special Issue underscores the importance of innovative molecular methods in advancing the state of the art in pathogen research and fostering more effective responses to infectious threats worldwide.

Dr. Lucía Galli
Prof. Dr. Guillermo Giovambattista
Guest Editors

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Keywords

  • genomic technologies
  • surveillance
  • epidemiology
  • antimicrobial resistance
  • one health

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Published Papers (1 paper)

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Research

14 pages, 762 KB  
Article
Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
by Guillermo Giovambattista, Aronggaowa Bao, Olivia Marcuzzi, Ariel Loza Vega, Juan Antonio Pereira Rico, Maria Florencia Ortega Masague, Liz Aurora Castro Rojas, Ruben Dario Martinez, Odalys Uffo Reinosa and Yoko Aida
Pathogens 2025, 14(10), 1005; https://doi.org/10.3390/pathogens14101005 - 4 Oct 2025
Viewed by 339
Abstract
Bovine leukemia virus (BLV), an oncogenic retrovirus of the genus Deltaretrovirus, causes enzootic bovine leukosis (EBL), the most prevalent neoplastic disease in cattle and a major source of economic loss. While BLV prevalence has been studied in commercial breeds, data on native Latin [...] Read more.
Bovine leukemia virus (BLV), an oncogenic retrovirus of the genus Deltaretrovirus, causes enzootic bovine leukosis (EBL), the most prevalent neoplastic disease in cattle and a major source of economic loss. While BLV prevalence has been studied in commercial breeds, data on native Latin American cattle remain limited. This study assessed BLV infection and proviral load in 244 animals from six native breeds: Argentine Creole (CrAr), Patagonian Argentine Creole (CrArPat), Pampa Chaqueño Creole (CrPaCh), Bolivian Creole from Cochabamba (CrCoch), Saavedreño Creole (CrSaa), and Siboney (Sib), sampled across Argentina, Bolivia, Paraguay, and Cuba. BLV-CoCoMo-qPCR-2 assay detected BLV provirus in 76 animals (31.1%), with a mean load of 9923 copies per 105 cells (range: 1–79,740). Infection rates varied significantly by breed (9.8% in CrAr to 83.8% in CrPaCh) and country (15.6% in Argentina to 83.8% in Paraguay) (p = 9.999 × 10−5). Among positives, 57.9% exhibited low proviral load (≤1000 copies), and 13.2% showed moderate levels (1001–9999), suggesting potential resistance to EBL progression. This is the first comprehensive report of BLV proviral load in Creole cattle across Latin America, offering novel epidemiological insights and highlighting the importance of native breeds in BLV surveillance. Full article
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