Special Issue "From Basic Research to New Tools and Challenges for the Genotoxicity Testing of Nanomaterials"

A special issue of Nanomaterials (ISSN 2079-4991).

Deadline for manuscript submissions: 24 December 2019.

Special Issue Editors

Guest Editor
Dr. Valérie FESSARD Website E-Mail
French Agency for Food, Environmental and Occupational Health and Safety, Laboratoire de Fougères, Unité Toxicologie des Contaminants, 10B rue C. Bourgelat, 35 306 Fougères Cedex, FRANCE
Interests: Toxicology, DNA damage, food contaminants
Guest Editor
Dr. Fabrice Nesslany Website E-Mail
Institut Pasteur de Lille, Service Toxicologie, 1, rue du professeur Calmette, 59 000 Lille, FRANCE
Interests: Toxicology, DNA damage, nanomaterials, air pollution, risk assessment

Special Issue Information

Dear Colleagues,

This Special Issue is open to contributions presenting studies on the genotoxicity of nanomaterials. The human population is exposed to a broad diversity of nanomaterials either manufactured or found naturally. Issues are regularly faced when addressing risk assessments for nanomaterials, and their increasing use in consumer products raises public health concerns. Although tremendous data have been published or provided during collaborative funded projects, conclusions on the genotoxicity of well-known nanomaterials are controversial, the key drivers involved in the genotoxic response of nanomaterials are still unclear, as are the underlying mechanisms. Moreover, the assay conditions for genotoxicity testing are still debated.

Papers reporting on the following are welcome: i) the role of the physico-chemical characteristics of nanomaterials (shape, size, protein corona, coating) including modifications occurring throughout their lifecycle as part of the genotoxic response; ii) investigation of the interference with in vitro genotoxicity assays including improved protocols or new methods to overcome this interference; iii) conditions for genotoxicity testing including the cell line(s) to be used, maximum dose/concentration and the method of nanomaterial dispersion; iv) proposals for nanomaterial reference controls; and finally v) the development of new tools as well as new approaches (grouping, ranking, safe(r)-by-design, read-across, etc.) to improve and facilitate genotoxicity testing.

Dr. Valérie FESSARD
Dr. Fabrice Nesslany
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Nanomaterials is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • in vitro and in vivo genotoxicity
  • mutagenicity
  • DNA damage
  • mechanism of action
  • reference material
  • new tools
  • interference
  • physico-chemical drivers of genotoxicity
  • lifecycle
  • conditions of genotoxicity assays

Published Papers (3 papers)

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Research

Open AccessArticle
In Vitro Genotoxicity of Polystyrene Nanoparticles on the Human Fibroblast Hs27 Cell Line
Nanomaterials 2019, 9(9), 1299; https://doi.org/10.3390/nano9091299 - 11 Sep 2019
Abstract
Several studies have provided information on environmental nanoplastic particles/debris, but the in vitro cyto-genotoxicity is still insufficiently characterized. The aim of this study is to analyze the effects of polystyrene nanoparticles (PNPs) in the Hs27 cell line. The viability of Hs27 cells was [...] Read more.
Several studies have provided information on environmental nanoplastic particles/debris, but the in vitro cyto-genotoxicity is still insufficiently characterized. The aim of this study is to analyze the effects of polystyrene nanoparticles (PNPs) in the Hs27 cell line. The viability of Hs27 cells was determined following exposure at different time windows and PNP concentrations. The genotoxic effects of the PNPs were evaluated by the cytokinesis-block micronucleus (CBMN) assay after exposure to PNPs. We performed ROS analysis on HS27 cells to detect reactive oxygen species at different times and treatments in the presence of PNPs alone and PNPs added to the Crocus sativus L. extract. The different parameters of the CBMN test showed DNA damage, resulting in the increased formation of micronuclei and nuclear buds. We noted a greater increase in ROS production in the short treatment times, in contrast, PNPs added to Crocus sativus extract showed the ability to reduce ROS production. Finally, the SEM-EDX analysis showed a three-dimensional structure of the PNPs with an elemental composition given by C and O. This work defines PNP toxicity resulting in DNA damage and underlines the emerging problem of polystyrene nanoparticles, which extends transversely from the environment to humans; further studies are needed to clarify the internalization process. Full article
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Open AccessArticle
In Vitro Analysis of the Effects of ITER-Like Tungsten Nanoparticles: Cytotoxicity and Epigenotoxicity in BEAS-2B Cells
Nanomaterials 2019, 9(9), 1233; https://doi.org/10.3390/nano9091233 - 30 Aug 2019
Abstract
Tungsten was chosen as a wall component to interact with the plasma generated by the International Thermonuclear Experimental fusion Reactor (ITER). Nevertheless, during plasma operation tritiated tungsten nanoparticles (W-NPs) will be formed and potentially released into the environment following a Loss-Of-Vacuum-Accident, causing occupational [...] Read more.
Tungsten was chosen as a wall component to interact with the plasma generated by the International Thermonuclear Experimental fusion Reactor (ITER). Nevertheless, during plasma operation tritiated tungsten nanoparticles (W-NPs) will be formed and potentially released into the environment following a Loss-Of-Vacuum-Accident, causing occupational or accidental exposure. We therefore investigated, in the bronchial human-derived BEAS-2B cell line, the cytotoxic and epigenotoxic effects of two types of ITER-like W-NPs (plasma sputtering or laser ablation), in their pristine, hydrogenated, and tritiated forms. Long exposures (24 h) induced significant cytotoxicity, especially for the hydrogenated ones. Plasma W-NPs impaired cytostasis more severely than the laser ones and both types and forms of W-NPs induced significant micronuclei formation, as shown by cytokinesis-block micronucleus assay. Single DNA strand breaks, potentially triggered by oxidative stress, occurred upon exposure to W-NPs and independently of their form, as observed by alkaline comet assay. After 24 h it was shown that more than 50% of W was dissolved via oxidative dissolution. Overall, our results indicate that W-NPs can affect the in vitro viability of BEAS-2B cells and induce epigenotoxic alterations. We could not observe significant differences between plasma and laser W-NPs so their toxicity might not be triggered by the synthesis method. Full article
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Open AccessArticle
Thermal Reduction of Graphene Oxide Mitigates Its In Vivo Genotoxicity Toward Xenopus laevis Tadpoles
Nanomaterials 2019, 9(4), 584; https://doi.org/10.3390/nano9040584 - 09 Apr 2019
Cited by 4
Abstract
The worldwide increase of graphene family materials raises the question of the potential consequences resulting from their release in the environment and future consequences on ecosystem health, especially in the aquatic environment in which they are likely to accumulate. Thus, there is a [...] Read more.
The worldwide increase of graphene family materials raises the question of the potential consequences resulting from their release in the environment and future consequences on ecosystem health, especially in the aquatic environment in which they are likely to accumulate. Thus, there is a need to evaluate the biological and ecological risk but also to find innovative solutions leading to the production of safer materials. This work focuses on the evaluation of functional group-safety relationships regarding to graphene oxide (GO) in vivo genotoxic potential toward X. laevis tadpoles. For this purpose, thermal treatments in H2 atmosphere were applied to produce reduced graphene oxide (rGOs) with different surface group compositions. Analysis performed indicated that GO induced disturbances in erythrocyte cell cycle leading to accumulation of cells in G0/G1 phase. Significant genotoxicity due to oxidative stress was observed in larvae exposed to low GO concentration (0.1 mg·L−1). Reduction of GO at 200 °C and 1000 °C produced a material that was no longer genotoxic at low concentrations. X-ray photoelectron spectroscopy (XPS) analysis indicated that epoxide groups may constitute a good candidate to explain the genotoxic potential of the most oxidized form of the material. Thermal reduction of GO may constitute an appropriate “safer-by-design” strategy for the development of a safer material for environment. Full article
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