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Open AccessArticle

NPs-TiO2 and Lincomycin Coexposure Induces DNA Damage in Cultured Human Amniotic Cells

1
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy
2
Department of Woman, Child and General and Special Surgery, University of Campania “Luigi Vanvitelli”, 80138 Napoli, Italy
3
Sant’ Anna e San Sebastiano Hospital, 81100 Caserta, Italy
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(11), 1511; https://doi.org/10.3390/nano9111511
Received: 29 July 2019 / Revised: 18 October 2019 / Accepted: 21 October 2019 / Published: 23 October 2019
Titanium dioxide nanoparticles (NPs-TiO2 or TiO2-NPs) have been employed in many commercial products such as medicines, foods and cosmetics. TiO2-NPs are able to carry antibiotics to target cells enhancing the antimicrobial efficiency; so that these nanoparticles are generally used in antibiotic capsules, like lincomycin, added as a dye. Lincomycin is usually used to treat pregnancy bacterial vaginosis and its combination with TiO2-NPs arises questions on the potential effects on fetus health. This study investigated the potential impact of TiO2-NPs and lincomycin co-exposure on human amniocytes in vitro. Cytotoxicity was evaluated with trypan blue vitality test, while genotoxic damage was performed by Comet Test, Diffusion Assay and RAPD-PCR for 48 and 72 exposure hours. Lincomycin exposure produced no genotoxic effects on amniotic cells, instead, the TiO2-NPs exposure induced genotoxicity. TiO2-NPs and lincomycin co-exposure caused significant increase of DNA fragmentation, apoptosis and DNA damage in amniocytes starting from 48 exposure hours. These results contribute to monitor the use of TiO2-NPs combined with drugs in medical application. The potential impact of antibiotics with TiO2-NPs during pregnancy could be associated with adverse effects on embryo DNA. The use of nanomaterials in drugs formulation should be strictly controlled in order to minimize risks. View Full-Text
Keywords: titanium dioxide nanoparticles; lincomycin; human amniotic cells; in vitro genotoxicity; apoptosis; DNA damage titanium dioxide nanoparticles; lincomycin; human amniotic cells; in vitro genotoxicity; apoptosis; DNA damage
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Mottola, F.; Iovine, C.; Santonastaso, M.; Romeo, M.L.; Pacifico, S.; Cobellis, L.; Rocco, L. NPs-TiO2 and Lincomycin Coexposure Induces DNA Damage in Cultured Human Amniotic Cells. Nanomaterials 2019, 9, 1511.

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