Next Article in Journal
Self-Healable Electro-Conductive Hydrogels Based on Core-Shell Structured Nanocellulose/Carbon Nanotubes Hybrids for Use as Flexible Supercapacitors
Previous Article in Journal
Convenient Real-Time Monitoring of the Contamination of Surface Ion Trap
Previous Article in Special Issue
NPs-TiO2 and Lincomycin Coexposure Induces DNA Damage in Cultured Human Amniotic Cells
Open AccessArticle

ToxTracker Reporter Cell Lines as a Tool for Mechanism-Based (Geno)Toxicity Screening of Nanoparticles—Metals, Oxides and Quantum Dots

1
Institute of Environmental Medicine, Karolinska Institutet, 171 77 Stockholm, Sweden
2
KTH Royal Institute of Technology, Division of Surface and Corrosion Science, Department of Chemistry, 100 44 Stockholm, Sweden
3
Toxys, 2333 CG Leiden, The Netherlands
4
Division Bioscience and Materials, RISE Research Institutes of Sweden, 111 21 Stockholm, Sweden
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(1), 110; https://doi.org/10.3390/nano10010110
Received: 28 November 2019 / Revised: 20 December 2019 / Accepted: 25 December 2019 / Published: 6 January 2020
The increased use of nanoparticles (NPs) requires efficient testing of their potential toxic effects. A promising approach is to use reporter cell lines to quickly assess the activation of cellular stress response pathways. This study aimed to use the ToxTracker reporter cell lines to investigate (geno)toxicity of various metal- or metal oxide NPs and draw general conclusions on NP-induced effects, in combination with our previous findings. The NPs tested in this study (n = 18) also included quantum dots (QDs) in different sizes. The results showed a large variation in cytotoxicity of the NPs tested. Furthermore, whereas many induced oxidative stress only few activated reporters related to DNA damage. NPs of manganese (Mn and Mn3O4) induced the most remarkable ToxTracker response with activation of reporters for oxidative stress, DNA damage, protein unfolding and p53-related stress. The QDs (CdTe) were highly toxic showing clearly size-dependent effects and calculations suggest surface area as the most relevant dose metric. Of all NPs investigated in this and previous studies the following induce the DNA damage reporter; CuO, Co, CoO, CdTe QDs, Mn, Mn3O4, V2O5, and welding NPs. We suggest that these NPs are of particular concern when considering genotoxicity induced by metal- and metal oxide NPs. View Full-Text
Keywords: nanotoxicology; genotoxicity; DNA damage; metal oxides; high throughput screening nanotoxicology; genotoxicity; DNA damage; metal oxides; high throughput screening
Show Figures

Figure 1

MDPI and ACS Style

McCarrick, S.; Cappellini, F.; Kessler, A.; Moelijker, N.; Derr, R.; Hedberg, J.; Wold, S.; Blomberg, E.; Odnevall Wallinder, I.; Hendriks, G.; Karlsson, H.L. ToxTracker Reporter Cell Lines as a Tool for Mechanism-Based (Geno)Toxicity Screening of Nanoparticles—Metals, Oxides and Quantum Dots. Nanomaterials 2020, 10, 110.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop