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Special Issue "Autofluorescence Spectroscopy and Imaging"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 30 September 2019

Special Issue Editor

Guest Editor
Dr. Anna Cleta Croce

Intitute of Molecular Genetics, National Research Council (IGM-CNR), c/o Department of Biology and Biotechnology, University of Pavia, Pavia, Italy
Website | E-Mail
Interests: photobiology; near UV-visible autofluorescence analysis; dysregulated metabolism; endogenous fluorophores; label free-real time diagnosis; fluid optical biopsy

Special Issue Information

Dear Colleagues,

Light induced emission from organic compounds was initially observed in the early 1800s, and was called “dispersive reflexion”, until George Stokes coined the new word, “fluorescence”, in 1852. Finally, it was named “autofluorescence” when it was detected using the newly developed fluorescence microscope early in 1900s. Since then, the ubiquitously presence in the whole life kingdom of the numerous fluorescing biomolecules and their relationship with normal or diseased conditions, in parallel to the technological advances, inspired so many unceasing studies, that it is impossible to describe them all. Within the various fields of studies for label free, in situ, real time analytical applications, biomedicine played an important role. This relied on quite a few endogenous fluorophores. For example, the loss or accumulation of collagen may account for the detection of cancer in multilayered epithelia or in disorders such as fibrosis, respectively; NADH and flavins reveal changes in the cell energy metabolism and redox state; lipofuscins reflect retinopathy, or more generally the gathering of hazardous oxidative events in metabolic disorders; and advanced glycation products in the skin reflect pathologies such as diabetes, cardiac or renal failure. In any case, their applications are far from being able to fully exploit the huge potential of autofluorescence, considering the advances in the analytical devices and methods, including time-resolved and multispectral imaging, microendoscopy, and procedures for label-free, even slide-free, timely, and cost effective automated diagnosis. Various, additional fluorescing biomolecules also suggest that beyond the progress in biomedicine, we cannot overlook the potential of autofluorescence applications in fields ranging from the surveillance of alimentary goods, to plant pathology and environment pollution.

Therefore, this Special Issue is aimed at attracting contributions on autofluorescence in its various aspects, not excluding technological development, to further promote the application of wide ranging label-free analytical procedures.

Dr. Anna Cleta Croce
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • Autofluorescence
  • Cultured cells
  • Animal tissues and organs
  • NADH
  • Flavin
  • Lipofuscin
  • Collagen
  • Porphyrin
  • Retinoids
  • Energy/lipid metabolism
  • Mitochondria
  • Oxidative stress
  • Plants
  • Food
  • Environment
  • Optical redox
  • Spectroscopy
  • Imaging and FLIM
  • Time resolved analysis
  • Multiphoton excitation

Published Papers (1 paper)

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Open AccessArticle
Autofluorescence Imaging Reflects the Nuclear Enlargement of Tumor Cells as well as the Cell Proliferation Ability and Aberrant Status of the p53, Ki-67, and p16 Genes in Colon Neoplasms
Molecules 2019, 24(6), 1106; https://doi.org/10.3390/molecules24061106
Received: 10 January 2019 / Revised: 14 March 2019 / Accepted: 15 March 2019 / Published: 20 March 2019
PDF Full-text (2008 KB) | HTML Full-text | XML Full-text
Background: Autofluorescence imaging (AFI) is useful for diagnosing colon neoplasms, but what affects the AFI intensity remains unclear. This study investigated the association between AFI and the histological characteristics, aberrant methylation status, and aberrant expression in colon neoplasms. Methods: Fifty-three patients with colorectal [...] Read more.
Background: Autofluorescence imaging (AFI) is useful for diagnosing colon neoplasms, but what affects the AFI intensity remains unclear. This study investigated the association between AFI and the histological characteristics, aberrant methylation status, and aberrant expression in colon neoplasms. Methods: Fifty-three patients with colorectal neoplasms who underwent AFI were enrolled. The AFI intensity (F index) was compared with the pathological findings and gene alterations. The F index was calculated using an image analysis software program. The pathological findings were assessed by the tumor crypt density, cell densities, and N/C ratio. The aberrant methylation of p16, E-cadherin, Apc, Runx3, and hMLH1 genes was determined by a methylation-specific polymerase chain reaction. The aberrant expression of p53 and Ki-67 was evaluated by immunohistochemical staining. Results: An increased N/C ratio, the aberrant expression of p53, Ki-67, and the altered methylation of p16 went together with a lower F index. The other pathological findings and the methylation status showed no association with the F index. Conclusions: AFI reflects the nuclear enlargement of tumor cells, the cell proliferation ability, and the altered status of cell proliferation-related genes, indicating that AFI is a useful and practical method for predicting the dysplastic grade of tumor cells and cell proliferation. Full article
(This article belongs to the Special Issue Autofluorescence Spectroscopy and Imaging)

Figure 1

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

  1. Author: Derrick Z.Y. Yong

Affiliation: A-Star, Singapore Institute of Manufacturing Technology, Precision Measurements Group, Singapore City, Singapore

  1. Author: Tilo Wünsch

Affiliation: Charité – Universitätsmedizin Berlin, Visceral and Transplantation Surgery, Berlin, Germany

  1. Author: Jose Manuel De La Rosa Vazquez

Affiliation: Instituto Politécnico Nacional, Unidad Zacatenco, Mexico City, Mexico

  1. Author: Darine Abi-Haidar

Affiliation: Universite Paris 7- Denis Diderot, Paris, France

  1. Author: Baharudin Abdullah

Affiliation: School of Medical Sciences - Universiti Sains Malaysia, Department of Otolaryngology-Head and Neck Surgery, Kubang Kerian, Malaysia

  1. Author: Klaus K.J. Hallfeldt

Affiliation: Ludwig-Maximilians-Universität München, Campus Innenstadt, Munich, Germany

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