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Autofluorescence Imaging Reflects the Nuclear Enlargement of Tumor Cells as well as the Cell Proliferation Ability and Aberrant Status of the p53, Ki-67, and p16 Genes in Colon Neoplasms

1
Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Asahikawa 078-8510, Japan
2
Digestive Disease Center, Asahikawa City Hospital, Asahikawa 070-8610, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Anna Cleta Croce
Molecules 2019, 24(6), 1106; https://doi.org/10.3390/molecules24061106
Received: 10 January 2019 / Revised: 14 March 2019 / Accepted: 15 March 2019 / Published: 20 March 2019
(This article belongs to the Special Issue Autofluorescence Spectroscopy and Imaging)
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Abstract

Background: Autofluorescence imaging (AFI) is useful for diagnosing colon neoplasms, but what affects the AFI intensity remains unclear. This study investigated the association between AFI and the histological characteristics, aberrant methylation status, and aberrant expression in colon neoplasms. Methods: Fifty-three patients with colorectal neoplasms who underwent AFI were enrolled. The AFI intensity (F index) was compared with the pathological findings and gene alterations. The F index was calculated using an image analysis software program. The pathological findings were assessed by the tumor crypt density, cell densities, and N/C ratio. The aberrant methylation of p16, E-cadherin, Apc, Runx3, and hMLH1 genes was determined by a methylation-specific polymerase chain reaction. The aberrant expression of p53 and Ki-67 was evaluated by immunohistochemical staining. Results: An increased N/C ratio, the aberrant expression of p53, Ki-67, and the altered methylation of p16 went together with a lower F index. The other pathological findings and the methylation status showed no association with the F index. Conclusions: AFI reflects the nuclear enlargement of tumor cells, the cell proliferation ability, and the altered status of cell proliferation-related genes, indicating that AFI is a useful and practical method for predicting the dysplastic grade of tumor cells and cell proliferation. View Full-Text
Keywords: autofluorescence imaging; colon neoplasm; N/C ratio; methylation; tumor cell proliferation autofluorescence imaging; colon neoplasm; N/C ratio; methylation; tumor cell proliferation
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Moriichi, K.; Fujiya, M.; Kobayashi, Y.; Murakami, Y.; Iwama, T.; Kunogi, T.; Sasaki, T.; Ijiri, M.; Takahashi, K.; Tanaka, K.; Sakatani, A.; Ando, K.; Nomura, Y.; Ueno, N.; Kashima, S.; Ikuta, K.; Tanabe, H.; Mizukami, Y.; Saitoh, Y.; Okumura, T. Autofluorescence Imaging Reflects the Nuclear Enlargement of Tumor Cells as well as the Cell Proliferation Ability and Aberrant Status of the p53, Ki-67, and p16 Genes in Colon Neoplasms. Molecules 2019, 24, 1106.

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