Special Issue "Microsystems for Point-of-Care Testing"

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B:Biology".

Deadline for manuscript submissions: 31 January 2020.

Special Issue Editor

Prof. Zulfiqur Ali
E-Mail Website
Guest Editor
School of Health & Life Sciences, Teesside University, Middlesbrough, Tees Valley, TS1 3BX, UK
Interests: micro and nanofabrication; microfluidics; point-of-care diagnostic; optical and electrochemical transduction

Special Issue Information

Dear Colleagues,

Point-of-care (POC) testing offers the opportunity to move healthcare away from the symptomatic treatment of diseases towards more predictive, preventive and personalised medicine. POC testing has advantages over centralised rapid analysis laboratories with no requirement for expensive capital infrastructure or staff with specialist technical expertise. These devices are applicable in a variety of settings including within primary, secondary and tertiary care as well as within low to medium income countries (LMICs). A wide range of advances—including in assays, transducers, microfluidics, and device fabrication, connected instrumentation and data analytics—have allowed the development of a variety of applications including for chronic and infectious disease conditions. These advances are reflected in an increasing number of scientific publications, patents and commercial products that demonstrate high sensitivity, selectivity and reliability, as well as fast, accurate, cost-effective and user-friendly assays.

On this research topic, we welcome review articles and original research papers aimed at the related key issues of basic research, materials development, system integration and data management with new POC diagnostic technologies in the frame of emerging and demanding clinical and biotechnological applications.

Prof. Zulfiqur Ali
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Microfluidics
  • Lab-on-a-Chip
  • Point-of-Care Diagnostics
  • Rapid Diagnostics
  • Cancer Detection
  • Infectious and Chronic Diseases
  • Connected Instrumentation
  • Data Analytics
  • Device Fabrication

Published Papers (3 papers)

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Research

Open AccessArticle
Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers
Micromachines 2019, 10(12), 876; https://doi.org/10.3390/mi10120876 (registering DOI) - 13 Dec 2019
Abstract
Quantifiable sensing of common microbes in chronic wounds has the potential to enable an objective assessment of wound healing for diagnostic applications. Sensing platforms should be robust, simple, and flexible to provide clinicians with a point-of-care tool. In this work, solution blow spun [...] Read more.
Quantifiable sensing of common microbes in chronic wounds has the potential to enable an objective assessment of wound healing for diagnostic applications. Sensing platforms should be robust, simple, and flexible to provide clinicians with a point-of-care tool. In this work, solution blow spun poly (lactic acid)/multiwalled carbon nanotube nanofiber composites are used to detect the presence and concentration of Pseudomonas putida in vitro using changes in impedance. Impedance microbiology (IM) is a well-documented diagnostic technique used in many applications, including cancer detection, tuberculosis screening and pregnancy tests. Twenty-four hour real-time measurements of the equivalent circuit of three culture media were taken with an inductance, capacitance, and resistance (LCR) meter. Variations in impedance were calculated to correspond to the growth of P. putida. Additionally, instantaneous measurements of bacterial cultures were taken over a one-minute time point to display the fast sensing of bacterial load via IM. This proof-of-concept shows that conductive solution blow spun fiber mats is a valid fabrication technique to develop in situ wound dressing impedance sensors. Study results indicate successful measurement and quantification of bacterial growth in this proof-of-concept study. Full article
(This article belongs to the Special Issue Microsystems for Point-of-Care Testing)
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Open AccessArticle
Automated Pre-Analytic Processing of Whole Saliva Using Magnet-Beating for Point-of-Care Protein Biomarker Analysis
Micromachines 2019, 10(12), 833; https://doi.org/10.3390/mi10120833 - 30 Nov 2019
Abstract
Saliva offers many advantages for point-of-care (PoC) diagnostic applications due to non-invasive, easy, and cost-effective methods of collection. However, the complex matrix with its non-Newtonian behavior and high viscosity poses handling challenges. Several tedious and long pre-analytic steps, incompatible with PoC use, are [...] Read more.
Saliva offers many advantages for point-of-care (PoC) diagnostic applications due to non-invasive, easy, and cost-effective methods of collection. However, the complex matrix with its non-Newtonian behavior and high viscosity poses handling challenges. Several tedious and long pre-analytic steps, incompatible with PoC use, are required to liquefy and homogenize saliva samples before protein analysis can be performed. We apply magnet-beating to reduce hands-on time and to simplify sample preparation. A magnet in a chamber containing the whole saliva is actuated inside a centrifugal microfluidic cartridge by the interplay of centrifugal and magnetic forces. Rigorous mixing, which homogenizes the saliva sample, is then initiated. Consequently, fewer manual steps are required to introduce the whole saliva into the cartridge. After 4 min of magnet-beating, the processed sample can be used for protein analysis. The viscosity of whole saliva has been reduced from 10.4 to 2.3 mPa s. Immunoassay results after magnet-beating for three salivary periodontal markers (MMP-8, MMP-9, TIMP-1) showed a linear correlation with a slope of 0.99 when compared to results of reference method treated samples. Conclusively, magnet-beating has been shown to be a suitable method for the pre-analytic processing of whole saliva for fully automated PoC protein analysis. Full article
(This article belongs to the Special Issue Microsystems for Point-of-Care Testing)
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Open AccessArticle
Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
Micromachines 2019, 10(11), 777; https://doi.org/10.3390/mi10110777 - 14 Nov 2019
Abstract
Considering the lack of official vaccines and medicines for Ebola virus infection, reliable diagnostic methods are necessary for the control of the outbreak and the spread of the disease. We developed a microfluidic-chip-based portable system for fast and parallel detection of four Ebola [...] Read more.
Considering the lack of official vaccines and medicines for Ebola virus infection, reliable diagnostic methods are necessary for the control of the outbreak and the spread of the disease. We developed a microfluidic-chip-based portable system for fast and parallel detection of four Ebola virus species. The system is based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and consists of four specific LAMP primers, a disc microfluidic chip, and a portable real-time fluorescence detector. It could specifically and parallelly distinguish four species of the Ebola virus after only one sampling, including the Zaire Ebola virus, the Sudan Ebola virus, the Bundibugyo Ebola virus, and the Tai Forest Ebola virus, without cross-contamination. The limit of detection was as small as 10 copies per reaction, while the total consumption of sample and reagent was 0.94 μL per reaction. The final results could be obtained in 50 min after one addition of sample and reagent mixture. This approach provides simplicity, high sensitivity, and multi-target parallel detection at a low cost, which could enable convenient and effective on-site detections of the Ebola virus in the outdoors, remote areas, and modern hospitals. Full article
(This article belongs to the Special Issue Microsystems for Point-of-Care Testing)
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