Next Article in Journal
Dynamics Behaviors of Droplet on Hydrophobic Surfaces Driven by Electric Field
Next Article in Special Issue
Automated Pre-Analytic Processing of Whole Saliva Using Magnet-Beating for Point-of-Care Protein Biomarker Analysis
Previous Article in Journal
Calibration Technique of a Curved Zoom Compound Eye Imaging System
Open AccessArticle

Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System

1
Department of Biomedical Engineering, the School of Medicine, Tsinghua University, Beijing 100084, China
2
Department of Precision Instrument, Tsinghua University, Beijing 100084, China
3
National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(11), 777; https://doi.org/10.3390/mi10110777
Received: 21 October 2019 / Revised: 8 November 2019 / Accepted: 11 November 2019 / Published: 14 November 2019
(This article belongs to the Special Issue Microsystems for Point-of-Care Testing)
Considering the lack of official vaccines and medicines for Ebola virus infection, reliable diagnostic methods are necessary for the control of the outbreak and the spread of the disease. We developed a microfluidic-chip-based portable system for fast and parallel detection of four Ebola virus species. The system is based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and consists of four specific LAMP primers, a disc microfluidic chip, and a portable real-time fluorescence detector. It could specifically and parallelly distinguish four species of the Ebola virus after only one sampling, including the Zaire Ebola virus, the Sudan Ebola virus, the Bundibugyo Ebola virus, and the Tai Forest Ebola virus, without cross-contamination. The limit of detection was as small as 10 copies per reaction, while the total consumption of sample and reagent was 0.94 μL per reaction. The final results could be obtained in 50 min after one addition of sample and reagent mixture. This approach provides simplicity, high sensitivity, and multi-target parallel detection at a low cost, which could enable convenient and effective on-site detections of the Ebola virus in the outdoors, remote areas, and modern hospitals. View Full-Text
Keywords: Ebola virus; detection; reverse transcription loop-mediated isothermal amplification; microfluidic chip Ebola virus; detection; reverse transcription loop-mediated isothermal amplification; microfluidic chip
Show Figures

Figure 1

MDPI and ACS Style

Lin, X.; Jin, X.; Xu, B.; Wang, R.; Fu, R.; Su, Y.; Jiang, K.; Yang, H.; Lu, Y.; Guo, Y.; Huang, G. Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System. Micromachines 2019, 10, 777.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop