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Molecular and Histological Approaches in Oral Tissue Regeneration and Diagnostics

A Special Issue of International Journal of Molecular Sciences (ISSN 1422-0067) belonging to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (20 February 2026) | Viewed by 1990

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Guest Editor
Department of Biomedical and Dental Sciences and Morphofunctional Imaging, Division of Medical Biotechnologies and Preventive Medicine, University of Messina, 98125 Messina, Italy
Interests: oral health; biomaterials; dental tissues; histology; immunofluorescence; dental materials
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Special Issue Information

Dear Colleagues,

The integration of molecular and histological approaches in dentistry has become increasingly critical for advancing our understanding of oral tissue regeneration and diagnostics. These methodologies enable us to delve into the intricate cellular and molecular processes underlying both the repair of dental tissues and the progression of oral diseases. Molecular techniques, ranging from genomics and proteomics to advanced imaging, provide detailed insights into the mechanisms of tissue regeneration, while histological analyses offer a direct view of the structural and morphological changes occurring within dental tissues. Together, these approaches facilitate the identification of key biomarkers and pathways involved in tissue healing, disease progression, and the response to therapeutic interventions. In this Special Issue, we invite contributions that explore innovative strategies combining molecular and histological methods. Topics of interest include but are not limited to, the study of dental stem cells, tissue engineering, the molecular pathogenesis of dental diseases, and the development of advanced diagnostic tools. We particularly welcome research that demonstrates how these techniques can be applied to improve clinical outcomes and personalize dental care.

Dr. Fabiana Nicita
Guest Editor

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Keywords

  • dentistry
  • histological analysis
  • immunofluorescence
  • molecular pathogenesis
  • tissue

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Published Papers (1 paper)

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Research

26 pages, 15174 KB  
Article
Analysis of MSX1, RYK, NFκB p65, and CCL4 Proteins and MSX2, RYK, and PTX3 Genes in Human Cleft Lip Tissue
by Mārtiņš Vaivads, Alise Elizabete Rone and Māra Pilmane
Int. J. Mol. Sci. 2025, 26(21), 10599; https://doi.org/10.3390/ijms262110599 - 30 Oct 2025
Viewed by 1580
Abstract
Human cleft lip morphopathogenesis is a complicated process involving multiple genes and proteins. Certain factors like muscle segment homeobox 1 (MSX1) and 2 (MSX2) as well as receptor-like tyrosine kinase (RYK) are important during lip embryogenesis, while others like nuclear factor kappa-B protein [...] Read more.
Human cleft lip morphopathogenesis is a complicated process involving multiple genes and proteins. Certain factors like muscle segment homeobox 1 (MSX1) and 2 (MSX2) as well as receptor-like tyrosine kinase (RYK) are important during lip embryogenesis, while others like nuclear factor kappa-B protein 65 (NFκB p65), C-C motif chemokine ligand 4 (CCL4), and pentraxin 3 (PTX3) regulate local inflammation and immunomodulation. The exact role of these factors in human cleft morphopathogenesis remains uncertain and limits the opportunity to improve cleft treatment and possible prophylaxis. Immunohistochemistry (IHC) for MSX1, RYK, NFκB p65, and CCL4 proteins and chromogenic in situ hybridization (CISH) for MSX2, RYK, and PTX3 genes were used to analyze postnatal human cleft lip tissue (15 patients) and control tissue (6 patients). The semiquantitative counting method was used to assess factor/gene-signal-containing cells. Statistical analysis was performed. IHC findings showed decreased MSX1, NFκB p65, and CCL4 proteins in cleft lip connective tissue and endothelium, while RYK protein was decreased only in cleft connective tissue. CISH showed increases in MSX2 and RYK gene-signal-containing cells in cleft lip tissue while PTX3 did not differ from controls. Multiple statistically significant correlations were calculated. The findings are discussed in detail to determine their significance in cleft lip morphopathogenesis. Full article
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