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Mechanisms in Biofilm Formation, Tolerance and Control: 3rd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 20 September 2026 | Viewed by 1135

Special Issue Editors


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Special Issue Information

Dear Colleagues,

Research on biofilms has progressed rapidly in the last two decades. The scientific community has come to understand many things about the specific biology of microbial biofilms through varied microscopic, physical, chemical, and molecular techniques. Such advances provided have fundamental insights for managing biofilms using different approaches. For problematic biofilms, some studies seek to prevent biofilm formation, others aim to develop antimicrobial agents to treat existing biofilms, and others aim to disrupt the polymeric ties that bind the biofilms together. It is now clear that the study of biofilms requires a multidisciplinary approach and that the knowledge on many of the aspects affecting biofilm formation and resistance remains superficial.

This Special Issue will cover recent advances in biofilm research, particularly mechanisms underlying biofilm formation and tolerance to antimicrobials. Studies on control approaches targeting specific biofilm mechanisms are within the scope of this Special Issue.

Dr. Manuel Simões
Dr. Lúcia Chaves Simões
Guest Editors

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Keywords

  • adhesion
  • antimicrobial action
  • antimicrobial resistance
  • biofilm development
  • biofilm matrix
  • biofilm structure
  • quorum sensing
  • motility
  • surfaces

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Published Papers (1 paper)

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Research

12 pages, 936 KB  
Article
Comparative Genomics Reveals Unique Genetic Determinants of Biofilm Formation in Campylobacter
by Yiping He, Gretchen Dykes, Heather Koppenhöfer, Joseph Capobianco and Chin-Yi Chen
Int. J. Mol. Sci. 2026, 27(6), 2543; https://doi.org/10.3390/ijms27062543 - 10 Mar 2026
Viewed by 304
Abstract
A biofilm is a complex microbial community that protects bacterial cells from various stressors, including harsh environmental conditions, antimicrobial treatments, and host immune responses. This protective capability enhances Campylobacter survival during food processing and storage and facilitates transmission to humans. Despite their importance, [...] Read more.
A biofilm is a complex microbial community that protects bacterial cells from various stressors, including harsh environmental conditions, antimicrobial treatments, and host immune responses. This protective capability enhances Campylobacter survival during food processing and storage and facilitates transmission to humans. Despite their importance, the molecular mechanisms underlying Campylobacter biofilm formation and its impact on pathogen persistence remain poorly understood. In this study, we characterized the biofilm-forming ability of 18 C. jejuni and C. coli strains isolated from retail meat and performed whole-genome sequencing and comparative genomic analysis to identify strain-specific genes contributing to biofilm formation and maintenance. Phenotypic analysis revealed that C. jejuni strains YH001 and YH027 exhibited the strongest biofilm-forming capacity, producing the highest biomass among all isolates. Phylogenetic analysis indicated a close genetic relationship between these two strains, while pangenome analysis identified 19 unique genes/proteins specific to these strains. Functional annotation indicated their critical roles in adhesion, extracellular matrix production, and stress response. These findings demonstrate strain-specific biofilm formation in Campylobacter and highlight genetic determinants that may serve as targets for novel therapeutic approaches and intervention strategies to disrupt biofilms, improve food safety, and reduce persistent infections. Full article
(This article belongs to the Special Issue Mechanisms in Biofilm Formation, Tolerance and Control: 3rd Edition)
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