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Horticulturae
Special Issues
Alternative Control of Fruit Phytopathogens Pre- and Postharvest
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Alternative Control of Fruit Phytopathogens Pre- and Postharvest

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Plant Pathology and Disease Management (PPDM)".

Deadline for manuscript submissions: closed (22 October 2024) | Viewed by 4495

Special Issue Editor

Dr. Ramsés Ramón González-Estrada

E-Mail Website
Guest Editor
División de Estudios de Posgrado e Investigación, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Avenida Tecnológico 2595, Col. Lagos del Country, Tepic 63175, Mexico
Interests: edible coatings; films; biopolymer characterization; biological control agents; fungi control; fruits; quality; alternative man-agement of fruits diseases; natural compounds
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Fruit production is important due to its impact on economic issues as well as the benefits associated with the consumption of fruit. However, the presence of phytopathogens represents a huge challenge in the fruit industry. Traditionally, pathogen control is achieved via the use of chemical fungicides; however, even though they can be effective, their extensive use has led to environmental and health issues.

The purpose of this Special Issue is to present and contribute to the dissemination of new and valuable knowledge for pathogen management. New contributions are expected regarding GRAS compounds, biological control agents, antimicrobial compounds of a plant origin, films and edible coatings, and emerging and innovative technologies for disease management at the pre- and post-harvest stages.

Dr. Ramsés Ramón González-Estrada
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fruits
  • quality
  • disease management
  • green compounds
  • nanotechnology
  • fungi
  • bacteria
  • packaging
  • emerging technologies
  • pre-harvest
  • post-harvest

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Published Papers (3 papers)

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Research

18 pages, 2662 KiB  
Article
Propolis Hydroalcoholic Extracts: Biochemical Characterization and Antifungal Efficacy
by Abderraouf Sadallah, Eugenio Aprea, Rudy Cignola, Andrea Caratti, Chiara Cordero, Andrea Angeli, Stefan Martens and Alessandra Di Francesco
Horticulturae 2025, 11(2), 122; https://doi.org/10.3390/horticulturae11020122 - 23 Jan 2025
Viewed by 1008
Abstract
The present study investigated the antifungal potential of hydroalcoholic extracts of propolis against the causal agent of grey mould, Botrytis cinerea, by in vitro and in vivo assays. Five different propolis from different Italian regions were subjected to hydroalcoholic extraction using different [...] Read more.
The present study investigated the antifungal potential of hydroalcoholic extracts of propolis against the causal agent of grey mould, Botrytis cinerea, by in vitro and in vivo assays. Five different propolis from different Italian regions were subjected to hydroalcoholic extraction using different ethanol concentrations and extraction methods. The preliminary bio-assay showed significant inhibitory effects on B. cinerea mycelial growth of propolis extracts obtained using 90% ethanol and subjected to sonication. The calculation of EC50 values, based on the demonstrated efficacy of non-volatile and volatile metabolites of propolis extracts, was useful to understand the main fraction involved in the antifungal activity of the samples and to perform the in vivo assay on grape and blueberry fruits. Three of the propolis extracts showed a high amount of genistein. Conversely, the other two propolis showed a fair amount of apigenin, caffeic acid, chrysin, ferulic acid, kaempferol, luteolin, p-coumaric acid and quercetin. From the volatile analysis of propolis, the main compounds detected were α-cadinol, α-eudesmol, calamenene, cadinol, benzyl acetate, benzyl benzoate and benzyl alcohol. The results suggest that propolis extracts have potential as an effective postharvest antifungal treatment, with varying degrees of efficacy depending on the extraction method and the type of propolis metabolites. Full article
(This article belongs to the Special Issue Alternative Control of Fruit Phytopathogens Pre- and Postharvest)
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19 pages, 5943 KiB  
Article
Effect of Volatile Compounds Emitted by an Endophytic Yeast Isolated from the Endemic Plant Echinopsis chiloensis against Botrytis cinerea
by Araceli Vidal, Paulo Castro, Freddy Navarro, Rodolfo Parada, Leonora Mendoza and Milena Cotoras
Horticulturae 2024, 10(9), 1005; https://doi.org/10.3390/horticulturae10091005 - 22 Sep 2024
Cited by 1 | Viewed by 1215
Abstract
Our research group isolated an endophyte yeast from the endemic plant Echinopsis chiloensis. This yeast, identified as Naganishia sp, produces volatile organic compounds (VOC) with antifungal activity against Botrytis cinerea. Due to the need for alternative control methods for this pathogen, [...] Read more.
Our research group isolated an endophyte yeast from the endemic plant Echinopsis chiloensis. This yeast, identified as Naganishia sp, produces volatile organic compounds (VOC) with antifungal activity against Botrytis cinerea. Due to the need for alternative control methods for this pathogen, the effect of VOC on B. cinerea was analyzed. On the fourth day of cultivation, in the presence of VOCs, the mycelial growth of B. cinerea stopped. VOCs inhibited 32.8% of the conidia germination and reduced sporulation by 70.6%. These compounds promoted the formation of infection cushions. VOCs caused damage to the cell wall and plasma membrane in B. cinerea. On the other hand, the volatile compounds induced oxidative stress, and led to membrane lipid peroxidation after 16 and 24 h of incubation in the presence of VOCs, because an increased level of malondialdehyde content was observed. These compounds exerted a fungistatic effect on B. cinerea. The volatile compounds emitted by Naganishia sp were identified by gas chromatography coupled with mass spectrometry as 3-methylbutyl acetate, ethylbenzene, 1-ethyl-3-methylbenzene, 2-phenylethanol, 2-heptanone and (5E)-6,10-dimethylundeca-5,9-dien-2-one. The most abundant volatile compound, 2-phenylethanol, inhibited the mycelial growth of B. cinerea with an IC50 value of 0.21 µL mL−1. The effect of this pure compound was also fungistatic. Finally, it was shown that volatile compounds decreased the severity of B. cinerea infection in tomatoes and grapes by 64.7 and 43,1%, respectively. This is the first report identifying volatile compounds emitted by a Naganishia sp. and describing their mode of action against B. cinerea. Full article
(This article belongs to the Special Issue Alternative Control of Fruit Phytopathogens Pre- and Postharvest)
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17 pages, 2551 KiB  
Article
Efficacy of Postharvest Application of Aureobasidium pullulans to Control White Haze on Apples and Effect on the Fruit Mycobiome
by Giulia Remolif, Giada Schiavon, Marco Garello and Davide Spadaro
Horticulturae 2024, 10(9), 927; https://doi.org/10.3390/horticulturae10090927 - 30 Aug 2024
Cited by 3 | Viewed by 1311
Abstract
White haze, an emerging disorder caused by extensive fungal growth on the apple surface, results in a compromised fruit quality and decreased marketability. The use of biological control agents could be an interesting tool to reduce its development. This work aimed to test [...] Read more.
White haze, an emerging disorder caused by extensive fungal growth on the apple surface, results in a compromised fruit quality and decreased marketability. The use of biological control agents could be an interesting tool to reduce its development. This work aimed to test the efficacy of two Aureobasidium pullulans strains (AP2 and PL5) in controlling white haze on stored apples. An in vivo trial was conducted by inoculating fruits with white haze causal agents (Entyloma belangeri, Golubevia pallescens, Tilletiopsis washingtonensis) and treating them with the antagonistic yeasts. Three commercial biofungicides were also included in the trial. Both A. pullulans strains, along with the Metschnikowia fructicola-based product, reduced white haze incidence after 110 days of storage at 1 ± 1 °C and after 7 days of shelf life. Furthermore, the effect of A. pullulans application on the fruit fungal microbiome was assessed. A significant impact of apple matrix and treatment on the mycobiome composition was observed. Analyses showed a good colonization of A. pullulans on the treated apples, both epiphytically and endophytically. A decrease in white haze-related fungi abundance was observed in the treated fruits. Additionally, a reduction of Ramularia spp. and modifications in the abundance of other fungal genera were detected after storage and shelf life. Full article
(This article belongs to the Special Issue Alternative Control of Fruit Phytopathogens Pre- and Postharvest)
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