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Detection and Control of Foodborne Pathogens in Food Supply Chain

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (31 December 2025) | Viewed by 3279

Special Issue Editor


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Guest Editor
Food Safety Department, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Turin, Italy
Interests: food microbiology; food safety
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

I invite you to publish the results of your research in this Special Issue entitled “Detection and Control of Foodborne Pathogens in Food Supply Chain”. Every day, we are engaged in fundamental activities to develop rapid and effective laboratory methods that can identify and enumerate pathogenic microorganisms in food. Furthermore, our work aims to validate methods capable of characterizing and comparing strains of pathogenic microorganisms to resolve food poisoning outbreaks and map the persistence of microorganisms dangerous to the health of the consumer along the agri-food chain. The publication of such studies on the detection and characterization of pathogenic microorganisms can lead to the sharing of laboratory methods with excellent performance and the availability of data that safety competent authorities can use to facilitate plans for consumer health protection.

Dr. Daniela Manila Bianchi
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food safety
  • foodborne outbreak
  • food safety analytical methods
  • characterization of foodborne pathogens
  • performance validation of analytical methods used in food safety
  • results of monitoring and surveillance of official plans for food safety
  • foodborne outbreak investigation

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Published Papers (2 papers)

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Research

26 pages, 8005 KB  
Article
Molecular Evidence of Clonal Salmonella Enteritidis Persistence in Poultry Cold-Chain Environments Under Environmental Stress
by Khaled S. Gazi, Wafa A. Alshehri, Alhanouf M. Alkhammash, Nada Alqadri, Fayez Saeed Bahwerth, Roua S. Baty, Nahlah N. Albakri, Ashjan F. Khalel, Tariq Abdulmutaleb Alpakistany and Mohammad Melebari
Foods 2025, 14(22), 3943; https://doi.org/10.3390/foods14223943 - 18 Nov 2025
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Abstract
Breakdown of cold-chain integrity drives the persistence of foodborne pathogens in poultry supply chains in warm, mountainous climates. This study used Al-Mandaq (Saudi Arabia) as a model to assess genetic diversity and contamination in bacteria from poultry storage units using 16S rRNA sequencing, [...] Read more.
Breakdown of cold-chain integrity drives the persistence of foodborne pathogens in poultry supply chains in warm, mountainous climates. This study used Al-Mandaq (Saudi Arabia) as a model to assess genetic diversity and contamination in bacteria from poultry storage units using 16S rRNA sequencing, VITEK 2, selective culturing, and ISSR/RAPD fingerprinting on 150 swabs. The Salmonella enterica complex comprised 15/29 isolates (51.7%), followed by Escherichia spp. 6/29 (20.7%) and Bacillus spp. 3/29 (10.3%). Five Salmonella serovars were identified: Enteritidis (8), Waycross (3), Minnesota (2), Typhimurium (1), and Dublin (1). S. Enteritidis accounted for 8/29 isolates (27.6%) and predominated among Salmonella in supermarket retail samples in Al-Mandaq. Combined ISSR and RAPD cluster analysis revealed highly clonal S. Enteritidis groupings, consistent with cross-contamination and prolonged survival in refrigeration equipment. In resource-limited settings, the combined ISSR and RAPD approach enhanced identification and differentiation of bacterial contamination sources within refrigeration equipment, providing superior strain-level discrimination compared to single-marker systems and improving epidemiological traceability of cross-contamination events. These results highlight the risk of clonal pathogen persistence in poultry cold-chain environments and the value of integrated molecular fingerprinting for surveillance in challenging climates. Full article
(This article belongs to the Special Issue Detection and Control of Foodborne Pathogens in Food Supply Chain)
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15 pages, 866 KB  
Article
Investigation of Listeria monocytogenes in Food in Northwestern Italy (2020–2024)
by Monica Pitti, Matteo Tavecchia, Angelo Romano, Simona Carrella, Giovanna Previto and Daniela Manila Bianchi
Foods 2025, 14(21), 3788; https://doi.org/10.3390/foods14213788 - 5 Nov 2025
Viewed by 1951
Abstract
Listeria monocytogenes is a foodborne pathogen of significant public health concern due to its high environmental resilience and ability to cause severe infections in vulnerable populations. The objective of the present study is to characterize foodborne strains of Listeria monocytogenes isolated between 2020 [...] Read more.
Listeria monocytogenes is a foodborne pathogen of significant public health concern due to its high environmental resilience and ability to cause severe infections in vulnerable populations. The objective of the present study is to characterize foodborne strains of Listeria monocytogenes isolated between 2020 and 2024 in northwestern Italy. Lm was detected through isolation, biochemical confirmation and molecular serogrouping. Next generation sequencing (NGS) analysis was used to characterize the strains in terms of virulence and antibiotic resistance. A total of 39 positive samples were identified from various food matrices, including meat products, fish, cheeses and ready-to-eat foods. The most frequently detected serogroups were IIc and IIa, with a notable presence of the highly virulent IVb group. Next-generation sequencing (NGS) was applied to all isolates, revealing the presence of virulence genes associated with the LIPI-1 island and internalins. In addition to pathogenicity islands, genes related to stress resistance (clpCEP, Gad A, GadB, GadC), biofilm production (agrA, flaA, degU, hfq) and sortase-mediated anchoring of surface protein (strA, strB) have been identified. The presence of antibiotic resistance genes was confirmed, with all isolates harboring the fosX gene. Moreover, four isolates exhibited resistance determinants against antibiotics belonging to two different classes: tetracyclines (tetM) and lincosamides (lsa(A)). Multilocus sequence typing (MLST) showed that clonal complex CC9 was the most prevalent among the isolates. Further, cgMLST and SNP analyses identified a principal cluster of closely related strains, which were isolated from meat products. These findings highlight the need for continuous surveillance of L. monocytogenes. Full article
(This article belongs to the Special Issue Detection and Control of Foodborne Pathogens in Food Supply Chain)
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