Next-Generation Approaches to Foodborne Pathogens: Genomics, Resistance, and Emerging Risks

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: 28 February 2026 | Viewed by 639

Special Issue Editors


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Guest Editor
Department of Sustainable Food Process, Faculty of Agriculture, Food and Environmental Sciences, Università Cattolica del Sacro Cuore, Piacenza, Italy
Interests: food microbiology; food safety; molecular biology of microorganisms; genomics; fermented foods

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Guest Editor
Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy
Interests: food safety; food microbiology; whole genome sequencing; antimicrobial resistance

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Guest Editor
Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy
Interests: food microbiology; predictive microbiology; fermented foods; food safety; biogenic amines; natural antimicrobials; bioprotective cultures

Special Issue Information

Dear Colleagues,

The global burden of foodborne illnesses continues to demand urgent scientific attention, particularly in light of increasing antimicrobial resistance (AMR), evolving food systems, and climate-driven shifts in pathogen ecology. Recent technological breakthroughs including whole-genome sequencing, metagenomics, transcriptomics, and proteomics have enabled researchers to study pathogens at unprecedented resolution. These approaches not only enhance surveillance and source tracking but also uncover the mechanisms of virulence, stress tolerance, and antimicrobial resistance that allow bacteria to persist in diverse food matrices and environments. Furthermore, smart detection technologies, such as CRISPR-based diagnostics and prediction models, are paving the way for rapid and precise pathogen monitoring. We particularly encourage submissions that take a systems biology, ecological, or predictive modeling perspective to understand pathogen behavior in complex food ecosystems.

This Special Issue aims to provide insights into the following:

  • foodborne pathogens behavior mechanisms and evolution;
  • gene regulation networks and dissemination routes of virulence and resistance genes;
  • biofilm formation and surface persistence in processing environments;
  • safety assessment of new and alternative food matrices;
  • development of robust, rapid, and field-deployable detection tools;
  • development of risk mitigation strategies in food models.

We particularly encourage submissions that take a systems biology, ecological, or predictive modeling perspective to understand pathogen behavior in complex food ecosystems.

We look forward to receiving your contributions.

Dr. Claudia Cortimiglia
Dr. Frédérique Pasquali
Dr. Giulia Tabanelli
Guest Editors

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • foodborne pathogens
  • antimicrobial resistance
  • whole-genome sequencing
  • mitigations strategies
  • virulence and resistance mechanisms
  • biofilm formation
  • predictive modeling for food safety

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Published Papers (2 papers)

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Research

26 pages, 10003 KB  
Article
Prevalence, Virulence and Antimicrobial Resistance of Vibrio cholerae in Aquatic Products and Aquaculture Environment in Shanghai
by Yingqi Li, Junjun Liu, Xin Yang, Weiqing Lan, Yong Zhao and Xiaohong Sun
Foods 2025, 14(22), 3824; https://doi.org/10.3390/foods14223824 (registering DOI) - 7 Nov 2025
Abstract
In this study, we isolated 214 Vibrio cholerae strains from aquatic (shrimp, crab, grass carp, and crucian carp) and their cultured environment in Shanghai, China. The virulence, serotype, and antimicrobial susceptibility were tested, and polymerase chain reaction (PCR) was used to detect antimicrobial [...] Read more.
In this study, we isolated 214 Vibrio cholerae strains from aquatic (shrimp, crab, grass carp, and crucian carp) and their cultured environment in Shanghai, China. The virulence, serotype, and antimicrobial susceptibility were tested, and polymerase chain reaction (PCR) was used to detect antimicrobial resistance genes. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) was employed for cluster analysis of the isolated strains. The results showed that V. cholerae was found in 47.9% (114/238) of aquatic samples, with the highest detection rate in shrimp (81.1%), and the detection rate was highest in summer (70.0%). Most of the strains were non-O1/O139 groups, and virulence genes rtxC and hap had the highest detection rates of 92.5% and 91.1%. Of the 214 isolates, 69.6% were multidrug-resistant (MDR). The resistance rate of V. cholerae to sulfamethoxazole, ampicillin, and erythromycin was 97.2%, 85.5%, and 70.1%, and that to imipenem, tetracyclines, and aminoglycosides was less than 5%. The MAR index ranged from 0.05 to 0.47. When V. cholerae was screened for antimicrobial resistance genes, β-lactams CARB, chloramphenicol floR, and sulfonamides sul2 were found in 19.6%, 7.9%, and 6.5% of isolates, respectively. The results of ERIC-PCR clustering showed that the isolates had a high degree of genetic diversity. The widespread distribution of virulent and MDR V. cholerae strains poses a potential threat to food safety and public health, calling for improved monitoring and control measures in the aquaculture industry. Full article
14 pages, 2856 KB  
Article
Genomic Landscape and Antimicrobial Resistance of Listeria monocytogenes in Retail Chicken in Qingdao, China
by Wei Wang, Yao Zhong, Juntao Jia, Lidan Ma, Yan Lu, Qiushui Wang, Lijuan Gao, Jijuan Cao, Yinping Dong, Qiuyue Zheng and Jing Xiao
Foods 2025, 14(18), 3260; https://doi.org/10.3390/foods14183260 - 19 Sep 2025
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Abstract
Listeria monocytogenes (L. monocytogenes) is an important foodborne pathogen that poses great risks to food safety and public health, and knowledge about its presence and diversity in potential sources is crucial for effectively tracking and controlling it in the food chain. [...] Read more.
Listeria monocytogenes (L. monocytogenes) is an important foodborne pathogen that poses great risks to food safety and public health, and knowledge about its presence and diversity in potential sources is crucial for effectively tracking and controlling it in the food chain. In this study, we investigated the prevalence, antimicrobial susceptibility, and genomic characteristics of Listeria monocytogenes (L. monocytogenes) collected from retail chicken meat samples in Qingdao, China, in 2022. A total of 38 (10.6%, 38/360) L. monocytogenes isolates were recovered from 360 retail chickens. All 38 isolates were classified into two lineages (I and II), three serogroups (IIa, IIb, IIc), eight sequence types (STs), eight clonal complexes (CCs), eight Sublineages (SLs) and nine cgMLSTs (CTs). ST121 and ST9 were the most prevalent STs in this study. The ST121 strains from China had heterogeneity with those from other countries, while the Chinese ST9 strains had homogeneity with those from other countries. One resistance cassette tet(M)-entS-msr(D) was identified in eight L2-SL121-ST121-CT13265 isolates, the genetic structure of which was identical to that of three reference genomes. All isolates carried the L. monocytogenes pathogenic island (LIPI)-1, with only one carrying LIPI-3 and three carrying LIPI-4. In addition, 11 isolates subtyped as L2-SL121-ST121-CT13265 were found to have a premature stop codon (PMSC) in the inlA gene in this study. Our data revealed the antimicrobial susceptibility, genomic characteristics and evolutionary relationships of L. monocytogenes in retail chicken in Qingdao, China. The characterization of genotypes, virulence, stress and antimicrobial markers of strains circulating in retail chicken in Qingdao, as described in this study, provides the opportunity to improve risk assessments of L. monocytogenes exposure. Full article
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