Biocatalytic Strategies for Sustainable Extraction and Production of Functional Food Ingredients

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Physics and (Bio)Chemistry".

Deadline for manuscript submissions: 20 August 2026 | Viewed by 1201

Special Issue Editors


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Guest Editor
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia
Interests: functional oligosaccharides; prebiotics; enzyme immobilization; agroindustry by-product valorization; bioprocess design; optimization and modelling; downstream processing

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Guest Editor
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia
Interests: applied enzymology; natural antioxidants; polyphenols; esters; food waste recovery; green extraction methods of bioactive compounds; bioprocess design; optimization and modelling

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Guest Editor
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia
Interests: biocatalysis; enzymatic biotransformation of flavonoids; modeling and optimization of bioprocesses; enzyme-assisted extraction; waste valorization

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Guest Editor
Innovation Center of Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia
Interests: enzyme immobilization; nanobiocatalysts; nanocellulose; proteases; lignin valorization

Special Issue Information

Dear Colleagues,

The growing demand for health-promoting food products and sustainable food production has intensified interest in enzymatic processes as selective, efficient, and environmentally friendly tools for the development of functional food ingredients. Enzymes, whether naturally derived or engineered, play a vital role in modifying, releasing, hydrolyzing, and synthesizing compounds from agri-food sources, particularly from waste and by-products, thereby enhancing the nutritional and physiological properties of various food systems.

This Special Issue, “Biocatalytic Strategies for Sustainable Extraction and Production of Functional Food Ingredients”, aims to highlight the exploration and design of novel enzymes for innovative enzymatic processes used in the production of functional components, such as oligosaccharides, peptides, polyphenols, rare sugars, and other biologically active molecules. Particular emphasis is placed on enzymatic methods that align with the principles of circular bioeconomy, green processing, and health-oriented innovation.

We invite original research articles and reviews addressing the following topics:

  • The characterization and application of immobilized, engineered, or novel microbial food-grade enzymes;
  • Enzyme-assisted extraction, hydrolysis, and structural modification of bioactive food compounds;
  • Optimization and scale-up of enzymatic processes for the production of functional ingredients;
  • Valorization of agri-food residues through enzyme-driven approaches;
  • In vitro and in vivo evaluation of the bioactivity, prebiotic potential, and health benefits of enzymatically obtained compounds.

By bringing together interdisciplinary perspectives, the aim of this Special Issue is to foster sustainable innovations in the development of food ingredients based on biocatalysis. We look forward to your valuable contributions and to advancing knowledge in this dynamic and impactful field.

Dr. Milica Simović
Dr. Marija Ćorović
Dr. Ana Milivojević
Dr. Katarina M. Banjanac
Guest Editors

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Keywords

  • biocatalysis 
  • enzyme engineering 
  • enzyme immobilization 
  • bioactive molecules 
  • prebiotics 
  • enzyme-assisted extraction 
  • waste and by-product valorization

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Published Papers (2 papers)

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Research

17 pages, 565 KB  
Article
Natural α-Amylase Inhibitors from Medicinal Herbs: In Vitro Evaluation of Extracts Prepared with Food-Compatible Solvents
by Mihailo Mladenović, Milica Milutinović, Nevena Đukić and Mirjana Rajilić-Stojanović
Foods 2026, 15(11), 1843; https://doi.org/10.3390/foods15111843 - 23 May 2026
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Abstract
Medicinal plants represent a promising source of bioactive compounds with potential antidiabetic activity, while the efficacy of plant extracts depends on both plant matrix and extraction conditions. This study aimed to systematically compare selected medicinal plants and extraction solvents to evaluate their impact [...] Read more.
Medicinal plants represent a promising source of bioactive compounds with potential antidiabetic activity, while the efficacy of plant extracts depends on both plant matrix and extraction conditions. This study aimed to systematically compare selected medicinal plants and extraction solvents to evaluate their impact on extracts’ in vitro α-amylase inhibitory activity, total polyphenol content (TPC), antioxidant capacity, and antimicrobial properties. Extracts of sage (Salvia officinalis), blueberry leaf (Vaccinium myrtillus), nettle (Urtica dioica), wormwood (Artemisia absinthium), and green and roasted coffee (Coffea arabica) were prepared using different solvent systems (50% (v/v) ethanol, propylene glycol, glycerol, and water), as well as a traditional aqueous infusion protocol. Extraction solvent strongly affected bioactivity: ethanol extracts showed the highest α-amylase inhibition, particularly in sage extract (79.60%) and blueberry leaf (57.71%). No significant correlation with TPC was observed (r = 0.229, p = 0.108), but aqueous ethanol yielded the highest TPC, with blueberry leaf being richest (64.16 ± 0.82 mg GAE/g), followed by roasted coffee (49.36 ± 0.83 mg GAE/g). Ethanol extracts demonstrated antibacterial activity against Staphylococcus aureus. Overall, sage and blueberry leaves showed the most promising multifunctional activity, highlighting their potential for further investigation as functional food ingredients. Full article
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21 pages, 2642 KB  
Article
Pectic Polysaccharides Recovery from Rapeseed Meal via Conventional and Enzyme-Assisted Extraction Techniques: Toward Emerging Prebiotic Pectic Oligosaccharide Development
by Katarina Banjanac, Milica Veljković, Milica Simović, Aleksandra Tomić, Paula López-Revenga, Antonia Montilla, Francisco Javier Moreno and Dejan Bezbradica
Foods 2026, 15(8), 1338; https://doi.org/10.3390/foods15081338 - 12 Apr 2026
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Abstract
This study investigates the extraction of pectic polysaccharides from rapeseed meal (RSM) using both conventional and enzyme-assisted techniques, and the obtained pectic polysaccharide fractions will be used later to produce prebiotic pectic oligosaccharides (POS). A two-step process was developed, involving enzymatic treatment with [...] Read more.
This study investigates the extraction of pectic polysaccharides from rapeseed meal (RSM) using both conventional and enzyme-assisted techniques, and the obtained pectic polysaccharide fractions will be used later to produce prebiotic pectic oligosaccharides (POS). A two-step process was developed, involving enzymatic treatment with Alcalase® 2.4 L for 2 h and Cellic® CTec3 HS preparations for 24 h, followed by ammonium oxalate extraction, which effectively isolated two pectic polysaccharide-enriched fractions: PP-EAE (first step) and the resulting Ca-bound pectic polysaccharides fraction (CaPP-EAE) (second step). Both fractions exhibited a bimodal molecular weight profile, indicative of the presence of long-chain polysaccharides alongside oligosaccharides. CaPP-EAE compositional analysis revealed that the fraction contained 56.8% galacturonic acid (GalA), low methyl-esterified (LM) pectins with 53.2% homogalacturonan (HG) and 30.2% rhamnogalacturonan I (RG-I) domains, featuring side chains of arabinan, arabinogalactan, and galactan. Subsequent enzymatic treatment with 0.5% (v/v) of Pectinex® Ultra Passover for 30 min transformed these fragments into a mixture of short-chain POS. Importantly, the produced short-chain POS fraction demonstrated enhanced prebiotic activity, particularly for bacterial strains of the family Lactobacillaceae, compared to a yeast strain. These findings provide a sustainable, biorefinery-compatible approach for extracting and modifying RSM polysaccharides, supporting the development of structurally defined POS as novel prebiotics. Full article
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