Special Issue "Enzymes and Biocatalysis"

A special issue of Catalysts (ISSN 2073-4344). This special issue belongs to the section "Biocatalysis".

Deadline for manuscript submissions: 31 October 2021.

Special Issue Editors

Assoc. Prof. Dr. Chia-Hung Kuo
Website1 Website2
Guest Editor
National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan
Interests: cellulase; lipase esterification and trans esterification; amylase; enzymatic kinetics; ultrasound-assisted enzymatic reaction; enzyme extraction; biotransformation; saccharification; response surface methodology; artificial neural network; wine fermentation
Special Issues and Collections in MDPI journals
Prof. Dr. Chun-Yung Huang
Website
Guest Editor
Prof. Dr. Chwen-Jen Shieh
Website
Guest Editor
Biotechnology Center, National Chung Hsing University, Taichung, Taiwan
Interests: biodiesel; lipid biocatalysis; enzyme technology; bioprocess optimization; supercritical fluid technology; Chinese herb medicine biotechnology
Prof. Dr. Cheng-Di Dong
Website1 Website2
Guest Editor
National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan
Interests: biocatalysis and biochar application; sustainable environment management; advanced oxidative and reductive water purification technology; principles and applications of environmental nanomaterials; sludge and groundwater investigation and remediation; water and wastewater treatment technology and water reuse

Special Issue Information

Dear Colleagues,

Enzymes are proteins produced by living cells to catalyze a reaction—so-called biocatalysts. Biocatalysis refers to the use of microorganisms and enzymes in chemical reactions, has become increasingly popular, and is frequently applied in the industry, since enzymes are highly specific and high catalytic efficiencies. The biocatalysis system offers several advantages in terms of improved reaction selectivity, reduced energy costs, enhanced reliability, environmental friendliness and sustainability.

In this Special Issue, we welcome original research articles and reviews focused on all aspects of enzymes used for biocatalysis, process optimization, ultrasonic processes, fine chemicals production, enzymatic assisted extraction, enzyme production, biocatalytic processes, environmental protection, bio-reactor, food process, biomass utilization, bioresource application, bio-transformations, enzymology, biological activity, the enzymatic synthesis of value-added chemicals, nanotechnology; environment and biodiversity, bioremediation, etc.

Assoc. Pro Chia-Hung Kuo
Prof. Dr. Chun-Yung Huang
Prof. Dr. Chwen-Jen Shieh
Prof. Dr. Cheng-Di Dong
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Catalysts is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • enzyme production
  • enzyme purification
  • biocatalysis
  • optimization
  • lipase
  • cellulase
  • protease
  • amylase
  • biofuels
  • enzymatic-catalyzed synthesis
  • enzyme immobilization
  • biomass utilization
  • environmental protection
  • glucosidase
  • biotransformation
  • enzymatic kinetics
  • ultrasound-assisted enzymatic reaction
  • bio-reactor
  • enzymatic-assisted extraction
  • experimental design
  • response surface methodology (RSM)
  • artificial neural network (ANN)

Published Papers

This special issue is now open for submission, see below for planned papers.

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: A lipase-packed-bed bioreactor for continuous synthesis of DHA and EPA ethyl esters
Authors: Chia-Hung Kuo
Affiliation: National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan
Abstract: The present study relates to a continuous process for synthesis of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) ethyl esters by using a lipase-packed-bed bioreactor. The DHA+EPA concentrate (in free fatty acid form) prepared by acetone fractionation of fatty acid salts method, contained 54.4% DHA and 16.8% EPA, was used as raw material. After pass through the lipase-packed-bed bioreactor with ethyl esters, the fatty acid was esterified to DHA and EPA ethyl esters. The use of continuous ultrasound-assisted packed-bed bioreactor for synthesis of DHA/EPA ethyl esters in a solvent free system was investigated. The kinetic and mass transfer studies demonstrated that ultrasound increases the contacts between substrates and enzyme. A three-level–two-factor central composite design and a response surface methodology (RSM) are employed to evaluate the effects of flow rate and DHA+EPA concentration on the percentage molar conversion of DHA+EPA ethyl ester. The conditions for ultrasound-assisted packed-bed bioreactor synthesis DHA/EPA ethyl ester were: flow rate of 5 mL/min, and DHA+EPA concentration of 500 mM, the molar conversion of DHA+EPA ethyl esters were 93.03%. The present study provides a method for continuous synthesis of DHA and EPA ethyl esters by acidolysis reaction using a lipase packed-bed bioreactor, and the continuous production can reduce the production cost and improve the efficiency and yield. The present study relates to a continuous process for synthesis of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) ethyl esters by using a lipase-packed-bed bioreactor. The DHA+EPA concentrate (in free fatty acid form) prepared by acetone fractionation of fatty acid salts method, contained 54.4% DHA and 16.8% EPA, was used as raw material. After pass through the lipase-packed-bed bioreactor with ethyl esters, the fatty acid was esterified to DHA and EPA ethyl esters. The use of continuous ultrasound-assisted packed-bed bioreactor for synthesis of DHA/EPA ethyl esters in a solvent free system was investigated. The kinetic and mass transfer studies demonstrated that ultrasound increases the contacts between substrates and enzyme. A three-level–two-factor central composite design and a response surface methodology (RSM) are employed to evaluate the effects of flow rate and DHA+EPA concentration on the percentage molar conversion of DHA+EPA ethyl ester. The conditions for ultrasound-assisted packed-bed bioreactor synthesis DHA/EPA ethyl ester were: flow rate of 5 mL/min, and DHA+EPA concentration of 500 mM, the molar conversion of DHA+EPA ethyl esters were 93.03%. The present study provides a method for continuous synthesis of DHA and EPA ethyl esters by acidolysis reaction using a lipase packed-bed bioreactor, and the continuous production can reduce the production cost and improve the efficiency and yield.

Back to TopTop