Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4
Abstract
:1. Introduction
2. Results
2.1. Expression and Purification of Recombinant BoPAL4 in Escherichia coli
2.2. Optimum pH and Temperature for PAL, TAL and DAL Activities of BoPAL4
2.3. Kinetic Parameters for PAL, TAL, and DAL Activities of BoPAL4
2.4. Kinetic Parameters for PAL Activity of BoPAL4-H123F
2.5. Comparison of Specific Activities in Wild-Type BoPAL4 and BoPAL4-H123F Mutants
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Bacterial Strains, Plasmids Construction, Site-Directed Mutagenesis, and Bacterial Growth Conditions
4.3. Preparations of BoPAL4 Enzymes
4.4. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
4.5. PAL Activity Assay
4.6. TAL Activity Assay
4.7. DAL Activity Assay
4.8. Biochemical Properties and Enzyme Kinetic
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Strain | Relevant Characteristics | Source or Ref. |
E. coli Top10 | F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ– rpsL(StrR) endA1 nupG | Invitrogen |
Plasmid | Relevant Characteristics | Source or Ref. |
pTrcHisA | E. coli expression vector with N-terminal His6-tag fusion | Invitrogen |
pTrcHisA-BoPAL4 | BoPAL4 coding sequence inserted into pTrcHisA | [27] |
pTrcHisA-BoPAL4-H123F | Point mutation H123F derivative of pTrcHisA-BoPAL4 | This study |
Protein | Substrate 1 | Optimum pH | Optimum Temp (°C) | kcat (s−1) | Km (μM) | kcat/Km (s−1 μM−1) |
---|---|---|---|---|---|---|
BoPAL4 | L-Phe | 9.0 | 50 | 1.44 | 2084 | 6.9 × 10−4 |
L-Tyr | 8.5 | 60 | 0.18 | 98 | 18.4 × 10−4 | |
L-DOPA | 9.0 | 40 | 0.06 | 956 | 0.6 × 10−4 | |
BoPAL4-H123F | L-Phe | 9.0 | 50 | 1.87 | 640 | 29.2 × 10−4 |
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Hsieh, C.-Y.; Huang, Y.-H.; Yeh, H.-H.; Hong, P.-Y.; Hsiao, C.-J.; Hsieh, L.-S. Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4. Catalysts 2021, 11, 1263. https://doi.org/10.3390/catal11111263
Hsieh C-Y, Huang Y-H, Yeh H-H, Hong P-Y, Hsiao C-J, Hsieh L-S. Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4. Catalysts. 2021; 11(11):1263. https://doi.org/10.3390/catal11111263
Chicago/Turabian StyleHsieh, Chun-Yen, Yi-Hao Huang, Hui-Hsuan Yeh, Pei-Yu Hong, Che-Jen Hsiao, and Lu-Sheng Hsieh. 2021. "Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4" Catalysts 11, no. 11: 1263. https://doi.org/10.3390/catal11111263