High Throughput Techniques for Enteric Viruses

Dear Colleagues,

Enteric viruses impose a considerable public health and economic burden globally. With numerous recent advances in multiple analytical techniques, the study of enteric viruses has been drastically affected. There is a need to compile and present the numerous applications of these techniques to the study, detection, and control of enteric viruses.

In the past decade, numerous advances in nucleic acid sequencing, alternative nucleic acid amplification, proteomic, bioinformatic, microfluidic, drug screening, imaging, and molecular editing techniques has drastically altered the way microbes are studied. Given the considerable importance of enteric viruses, the application of these techniques to enteric viruses can have—and has had—dramatic effects. For instance, the application of a high throughput CRISPR-Cas9 technique to screen host genes essential for viral replication has allowed for the identification of host cell receptors. Multiple bioinformatic pipelines for detection and identification of viruses in metagenomics have been reported and help inform multiple characteristics of the viruses related to their evolution and transmission. Multiple advances in microfluidics has allowed for lab-on-a-chip technologies to potentially detect enteric viruses with minimal sample preparation. Application of proteomics techniques has helped inform the study of enteric viral replication and host response. Advancements in multiplexing and isothermal amplification techniques has created the potential for truly portable “lab-in-a-suitcase” assays for rapid enteric viral detection. This Special Issue on “High Throughput Techniques for Enteric Viruses” will focus on the utilization and application of next generation high throughput techniques to enteric viruses from basic study of viral pathogenesis to translational application. Manuscripts describing utilization or application of high throughput, next generation: sequencing and bioinformatic, proteomic, microfluidic, molecular editing, drug screening, imaging, nucleic acid amplification, metabolomic, and microarray techniques to viruses that replicate in the intestine are welcome.

Dr. Matthew D. Moore
Dr. Naim Montazeri
Guest Editor

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