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Biosensors
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Immunosensors 2012
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Immunosensors 2012

A special issue of Biosensors (ISSN 2079-6374).

Deadline for manuscript submissions: closed (31 December 2012) | Viewed by 64456

Special Issue Editor

Prof. Dr. Nicole Jaffrezic-Renault

E-Mail Website
Guest Editor
Institute of Analytical Sciences, UMR CNRS 5280, Department LSA, 5 Rue de La Doua, 69100 Villeurbanne, France
Interests: biosensors; impedance; immunosensors; conductometric sensors; enzymatic sensors; affinity sensors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This special issue will be devoted to general label-free affinity sensors. Different types of receptors could be included: Antibodies; Receptors; DNA and RNA Aptamers; Biomimetic receptors: molecularly imprinted polymers. All type of label-free transduction could be addressed (electrochemical, optical, acoustic…). Amplification of signal using nanoparticles, CNTs, quantum dots… should be highly appreciated. Multiarray of micro/nanotransducers will be preferred.
All types of application domains are acceptable.

Prof. Dr. Nicole Jaffrezic-Renault
Guest Editor

Keywords

  • antibodies
  • receptors
  • DNA and RNA aptamers
  • biomimetic receptors: molecularly imprinted polymers
  • label-free transduction (electrochemical, optical, acoustic…)
  • nanoparticles, CNTs, quantum dots
  • multiarray of micro/nanotransducers

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Published Papers (7 papers)

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Research

706 KiB  
Article
Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging
by Alina Karabchevsky, Lev Tsapovsky, Robert S. Marks and Ibrahim Abdulhalim
Biosensors 2013, 3(1), 157-170; https://doi.org/10.3390/bios3010157 - 19 Mar 2013
Cited by 25 | Viewed by 9351
Abstract
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the [...] Read more.
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor—estrone—was attached and detected in deionized water with a diverging beam SPR imaging sensor. Full article
(This article belongs to the Special Issue Immunosensors 2012)
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662 KiB  
Article
Fiber-Optic Fluoroimmunoassay System with a Flow-Through Cell for Rapid On-Site Determination of Escherichia coli O157:H7 by Monitoring Fluorescence Dynamics
by Kumiko Miyajima, Tomoyuki Koshida, Takahiro Arakawa, Hiroyuki Kudo, Hirokazu Saito, Kazuyoshi Yano and Kohji Mitsubayashi
Biosensors 2013, 3(1), 120-131; https://doi.org/10.3390/bios3010120 - 8 Mar 2013
Cited by 12 | Viewed by 9972
Abstract
Dynamic fluoroimmunoassay with a flow-through system using optical fiber probes consisting of polystyrene was developed and applied to a quantitative detection of E. coli O157:H7. The system measures E. coli as fluorescence of sandwich-type immune complexes formed by capture antibodies immobilized on the [...] Read more.
Dynamic fluoroimmunoassay with a flow-through system using optical fiber probes consisting of polystyrene was developed and applied to a quantitative detection of E. coli O157:H7. The system measures E. coli as fluorescence of sandwich-type immune complexes formed by capture antibodies immobilized on the surface of the probe, E. coli cells, and fluorescently labeled detection antibodies. Excitation was carried out using an evanescent wave from the probe. Resulting fluorescence recoupled into the probe was detected by a photodiode. The assay system was constructed with a flow cell which was available for sequential injection of experimental reagents. In vitro characterization was performed using the flow cell, and the calibration range of E. coli O157:H7 was from 103 to 107 cells/mL. The measurement for each sample was completed within 12 min. Furthermore, it was also possible to estimate the concentrations of E. coli O157:H7 by the increasing rate of fluorescence during binding reaction of detection antibodies to antigens. This minimized the time for measurement down to 6 min. The system is suitable for rapid and direct determination for microorganisms or bacteria in food, clinical, and environmental sources. Full article
(This article belongs to the Special Issue Immunosensors 2012)
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377 KiB  
Article
Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
by Christian M. Winterflood, Thomas Ruckstuhl and Stefan Seeger
Biosensors 2013, 3(1), 108-115; https://doi.org/10.3390/bios3010108 - 8 Feb 2013
Cited by 3 | Viewed by 6665
Abstract
We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked [...] Read more.
We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements. Full article
(This article belongs to the Special Issue Immunosensors 2012)
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377 KiB  
Article
Single Step Nanoplasmonic Immunoassay for the Measurement of Protein Biomarkers
by Shradha Prabhulkar, Adam De la Zerda, Amit Paranjape and Richard M. Awdeh
Biosensors 2013, 3(1), 77-88; https://doi.org/10.3390/bios3010077 - 6 Feb 2013
Cited by 1 | Viewed by 7122
Abstract
A nanoplasmonic biosensor for highly-sensitive, single-step detection of protein biomarkers is presented. The principle is based on the utilization of the optical scattering properties of gold nanorods (GNRs) conjugated to bio-recognition molecules. The nanoplasmonic properties of the GNRs were utilized to detect proteins [...] Read more.
A nanoplasmonic biosensor for highly-sensitive, single-step detection of protein biomarkers is presented. The principle is based on the utilization of the optical scattering properties of gold nanorods (GNRs) conjugated to bio-recognition molecules. The nanoplasmonic properties of the GNRs were utilized to detect proteins using near-infrared light interferometry. We show that the antibody-conjugated GNRs can specifically bind to our model analyte, Glucose Transporter-1 (Glut-1). The signal intensity of back-scattered light from the GNRs bound after incubation, correlated well to the Glut-1 concentration as per the calibration curve. The detection range using this nanoplasmonic immunoassay ranges from 10 ng/mL to 1 ug/mL for Glut-1. The minimal detectable concentration based on the lowest discernable concentration from zero is 10 ng/mL. This nanoplasmonic immunoassay can act as a simple, selective, sensitive strategy for effective disease diagnosis. It offers advantages such as wide detection range, increased speed of analysis (due to fewer incubation/washing steps), and no label development as compared to traditional immunoassay techniques. Our future goal is to incorporate this detection strategy onto a microfluidic platform to be used as a point-of-care diagnostic tool. Full article
(This article belongs to the Special Issue Immunosensors 2012)
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847 KiB  
Article
Evaluating Inhibition of the Epidermal Growth Factor (EGF)-Induced Response of Mutant MCF10A Cells with an Acoustic Sensor
by Marcela P. Garcia, Ammar Shahid, Jennifer Y. Chen and Jun Xi
Biosensors 2012, 2(4), 448-464; https://doi.org/10.3390/bios2040448 - 13 Nov 2012
Cited by 5 | Viewed by 7096
Abstract
Many cancer treatments rely on inhibition of epidermal growth factor (EGF)-induced cellular responses. Evaluating drug effects on such responses becomes critical to the development of new cancer therapeutics. In this report, we have employed a label-free acoustic sensor, the quartz crystal microbalance with [...] Read more.
Many cancer treatments rely on inhibition of epidermal growth factor (EGF)-induced cellular responses. Evaluating drug effects on such responses becomes critical to the development of new cancer therapeutics. In this report, we have employed a label-free acoustic sensor, the quartz crystal microbalance with dissipation monitoring (QCM-D), to track the EGF-induced response of mutant MCF10A cells under various inhibitory conditions. We have identified a complex cell de-adhesion process, which can be distinctly altered by inhibitors of signaling pathways and cytoskeleton formation in a dose-dependent manner. The dose dependencies of the inhibitors provide IC50 values which are in strong agreement with the values reported in the literature, demonstrating the sensitivity and reliability of the QCM-D as a screening tool. Using immunofluorescence imaging, we have also verified the quantitative relationship between the ΔD-response (change in energy dissipation factor) and the level of focal adhesions quantified with the areal density of immunostained vinculin under those inhibitory conditions. Such a correlation suggests that the dynamic restructuring of focal adhesions can be assessed based on the time-dependent change in ΔD-response. Overall, this report has shown that the QCM-D has the potential to become an effective sensing platform for screening therapeutic agents that target signaling and cytoskeletal proteins. Full article
(This article belongs to the Special Issue Immunosensors 2012)
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392 KiB  
Article
A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well
by Caroline Mille, Dominique Debarnot, Willy Zorzi, Benaissa El Moualij, Isabelle Quadrio, Armand Perret-Liaudet, Arnaud Coudreuse, Gilbert Legeay and Fabienne Poncin-Epaillard
Biosensors 2012, 2(4), 433-447; https://doi.org/10.3390/bios2040433 - 24 Oct 2012
Viewed by 6864
Abstract
This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks [...] Read more.
This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation. Full article
(This article belongs to the Special Issue Immunosensors 2012)
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Graphical abstract

363 KiB  
Article
An Electrochemical Immunosensor for Detection of Staphylococcus aureus Bacteria Based on Immobilization of Antibodies on Self-Assembled Monolayers-Functionalized Gold Electrode
by Mohamed Braiek, Karima Bekir Rokbani, Amani Chrouda, Béchir Mrabet, Amina Bakhrouf, Abderrazak Maaref and Nicole Jaffrezic-Renault
Biosensors 2012, 2(4), 417-426; https://doi.org/10.3390/bios2040417 - 16 Oct 2012
Cited by 87 | Viewed by 16362
Abstract
The detection of pathogenic bacteria remains a challenge for the struggle against biological weapons, nosocomial diseases, and for food safety. In this research, our aim was to develop an easy-to-use electrochemical immunosensor for the detection of pathogenic Staphylococcus aureus ATCC25923. The biosensor was [...] Read more.
The detection of pathogenic bacteria remains a challenge for the struggle against biological weapons, nosocomial diseases, and for food safety. In this research, our aim was to develop an easy-to-use electrochemical immunosensor for the detection of pathogenic Staphylococcus aureus ATCC25923. The biosensor was elaborated by the immobilization of anti-S. aureus antibodies using a self-assembled monolayer (SAMs) of 3-Mercaptopropionic acid (MPA). These molecular assemblies were spontaneously formed by the immersion of the substrate in an organic solvent containing the SAMs that can covalently bond to the gold surface. The functionalization of the immunosensor was characterized using two electrochemical techniques: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Here, the analysis was performed in phosphate buffer with ferro/ferricyanide as the redox probe. The EIS technique was used for affinity assays: antibody-cell binding. A linear relationship between the increment in the electron transfer resistance (RCT) and the logarithmic value of S. aureus concentration was observed between 10 and 106 CFU/mL. The limit of detection (LOD) was observed at 10 CFU/mL, and the reproducibility was calculated to 8%. Finally, a good selectivity versus E. coli and S. epidermidis was obtained for our developed immunosensor demonstrating its specificity towards only S. aureus. Full article
(This article belongs to the Special Issue Immunosensors 2012)
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