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Open AccessArticle

Evaluating Inhibition of the Epidermal Growth Factor (EGF)-Induced Response of Mutant MCF10A Cells with an Acoustic Sensor

1
Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USA
2
Department of Biology, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USA
*
Author to whom correspondence should be addressed.
Biosensors 2012, 2(4), 448-464; https://doi.org/10.3390/bios2040448
Received: 29 August 2012 / Revised: 8 October 2012 / Accepted: 7 November 2012 / Published: 13 November 2012
(This article belongs to the Special Issue Immunosensors 2012)
Many cancer treatments rely on inhibition of epidermal growth factor (EGF)-induced cellular responses. Evaluating drug effects on such responses becomes critical to the development of new cancer therapeutics. In this report, we have employed a label-free acoustic sensor, the quartz crystal microbalance with dissipation monitoring (QCM-D), to track the EGF-induced response of mutant MCF10A cells under various inhibitory conditions. We have identified a complex cell de-adhesion process, which can be distinctly altered by inhibitors of signaling pathways and cytoskeleton formation in a dose-dependent manner. The dose dependencies of the inhibitors provide IC50 values which are in strong agreement with the values reported in the literature, demonstrating the sensitivity and reliability of the QCM-D as a screening tool. Using immunofluorescence imaging, we have also verified the quantitative relationship between the ΔD-response (change in energy dissipation factor) and the level of focal adhesions quantified with the areal density of immunostained vinculin under those inhibitory conditions. Such a correlation suggests that the dynamic restructuring of focal adhesions can be assessed based on the time-dependent change in ΔD-response. Overall, this report has shown that the QCM-D has the potential to become an effective sensing platform for screening therapeutic agents that target signaling and cytoskeletal proteins. View Full-Text
Keywords: QCM-D; biosensors; label free; EGFR; cell adhesion; cell signaling; cytoskeleton; inhibition; cancer development; drug screening QCM-D; biosensors; label free; EGFR; cell adhesion; cell signaling; cytoskeleton; inhibition; cancer development; drug screening
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Garcia, M.P.; Shahid, A.; Chen, J.Y.; Xi, J. Evaluating Inhibition of the Epidermal Growth Factor (EGF)-Induced Response of Mutant MCF10A Cells with an Acoustic Sensor. Biosensors 2012, 2, 448-464.

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