Fiber-Optic Fluoroimmunoassay System with a Flow-Through Cell for Rapid On-Site Determination of Escherichia coli O157:H7 by Monitoring Fluorescence Dynamics
Abstract
:1. Introduction
2. Experimental
2.1. Reagents
2.2. Construction of Flow-Through System for E. coli O157:H7 Detection
2.3. Characterization of the Flow-Through System
3. Results and Discussion
3.1. Measurement of E. coli O157:H7
3.2. Reproducibility of the Fluoroimmunosensor
3.3. Fluorescence Monitoring and Measuring of E. coli O157:H7 by the Flow-Through System
3.4. Selectivity of the Fluoroimmunosensor for E. coli O157:H7
4. Conclusions
Acknowledgments
References
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Miyajima, K.; Koshida, T.; Arakawa, T.; Kudo, H.; Saito, H.; Yano, K.; Mitsubayashi, K. Fiber-Optic Fluoroimmunoassay System with a Flow-Through Cell for Rapid On-Site Determination of Escherichia coli O157:H7 by Monitoring Fluorescence Dynamics. Biosensors 2013, 3, 120-131. https://doi.org/10.3390/bios3010120
Miyajima K, Koshida T, Arakawa T, Kudo H, Saito H, Yano K, Mitsubayashi K. Fiber-Optic Fluoroimmunoassay System with a Flow-Through Cell for Rapid On-Site Determination of Escherichia coli O157:H7 by Monitoring Fluorescence Dynamics. Biosensors. 2013; 3(1):120-131. https://doi.org/10.3390/bios3010120
Chicago/Turabian StyleMiyajima, Kumiko, Tomoyuki Koshida, Takahiro Arakawa, Hiroyuki Kudo, Hirokazu Saito, Kazuyoshi Yano, and Kohji Mitsubayashi. 2013. "Fiber-Optic Fluoroimmunoassay System with a Flow-Through Cell for Rapid On-Site Determination of Escherichia coli O157:H7 by Monitoring Fluorescence Dynamics" Biosensors 3, no. 1: 120-131. https://doi.org/10.3390/bios3010120
APA StyleMiyajima, K., Koshida, T., Arakawa, T., Kudo, H., Saito, H., Yano, K., & Mitsubayashi, K. (2013). Fiber-Optic Fluoroimmunoassay System with a Flow-Through Cell for Rapid On-Site Determination of Escherichia coli O157:H7 by Monitoring Fluorescence Dynamics. Biosensors, 3(1), 120-131. https://doi.org/10.3390/bios3010120