An Electrochemical Immunosensor for Detection of Staphylococcus aureus Bacteria Based on Immobilization of Antibodies on Self-Assembled Monolayers-Functionalized Gold Electrode
Abstract
:1. Introduction
2. Experimental
2.1. Reagents
2.2. Bacteria Cultivation
2.3. Instrumentation and Techniques
2.4. Elaboration of the Immunosensor
3. Results and Discussion
3.1. Characterization of the Functionalized Electrode
3.2. Impedance Measurements for the Detection of S. aureus Bacteria
- -
- A resistance related to the charge transfer rate of the redox reaction at the biofunctionalized electrode; RCT.
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- A constant phase element (CPE) that is related to the capacitance of the biofunctionalized Au electrode/electrolyte interface. CPE reflects the non-ideality of the double-layer at the biofunctionalized gold electrode/electrolyte interface due to the roughness and porosity of the biofilm.
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- A specific electrochemical element of diffusion, the Warburg element (Zw).
Type of transducer | Detection Limit | Dynamic range | Reference |
---|---|---|---|
Impedance | 10 CFU/mL | 10–106 CFU/mL | This work |
Impedance | 102 CFU/mL | 102–107 CFU/mL | [11] |
Amperometry | 3.7 × 102 CFU/mL | 1.3 × 103–7.6 × 104 CFU/mL | [10] |
SPR | 105 CFU/mL | - | [9] |
4. Conclusion
Acknowledgments
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Braiek, M.; Rokbani, K.B.; Chrouda, A.; Mrabet, B.; Bakhrouf, A.; Maaref, A.; Jaffrezic-Renault, N. An Electrochemical Immunosensor for Detection of Staphylococcus aureus Bacteria Based on Immobilization of Antibodies on Self-Assembled Monolayers-Functionalized Gold Electrode. Biosensors 2012, 2, 417-426. https://doi.org/10.3390/bios2040417
Braiek M, Rokbani KB, Chrouda A, Mrabet B, Bakhrouf A, Maaref A, Jaffrezic-Renault N. An Electrochemical Immunosensor for Detection of Staphylococcus aureus Bacteria Based on Immobilization of Antibodies on Self-Assembled Monolayers-Functionalized Gold Electrode. Biosensors. 2012; 2(4):417-426. https://doi.org/10.3390/bios2040417
Chicago/Turabian StyleBraiek, Mohamed, Karima Bekir Rokbani, Amani Chrouda, Béchir Mrabet, Amina Bakhrouf, Abderrazak Maaref, and Nicole Jaffrezic-Renault. 2012. "An Electrochemical Immunosensor for Detection of Staphylococcus aureus Bacteria Based on Immobilization of Antibodies on Self-Assembled Monolayers-Functionalized Gold Electrode" Biosensors 2, no. 4: 417-426. https://doi.org/10.3390/bios2040417
APA StyleBraiek, M., Rokbani, K. B., Chrouda, A., Mrabet, B., Bakhrouf, A., Maaref, A., & Jaffrezic-Renault, N. (2012). An Electrochemical Immunosensor for Detection of Staphylococcus aureus Bacteria Based on Immobilization of Antibodies on Self-Assembled Monolayers-Functionalized Gold Electrode. Biosensors, 2(4), 417-426. https://doi.org/10.3390/bios2040417