Aptamer-Based Biosensors for Point-of-Care Diagnostics—2nd Edition

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 30 December 2026 | Viewed by 1243

Special Issue Editors


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Guest Editor
Department of Analytical Chemistry, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 4 Pasteur Street, 400349 Cluj-Napoca, Romania
Interests: electrochemical and optical sensors; graphene; nanomaterials based electrodes; bioanalysis
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Guest Editor
Departamento de Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006 Oviedo, Spain
Interests: biosensors; electrochemistry; cancer; aptamers; glycans
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The fast-changing fields of molecular diagnostics and personalized medicine are driving demand for innovative, rapid, and highly sensitive point-of-care (POC) technologies. Aptamer-based biosensors have become one of the most versatile and powerful options. These synthetic nucleic acid ligands provide exceptional binding precision, enabling next-generation POC devices capable of detecting a wide range of biomarkers. Their programmability, robustness, and compatibility with diverse sensing formats place them at the forefront of advances in clinical diagnostics, infectious disease monitoring, environmental analysis, and personalized healthcare.

Building on the momentum of the previous edition, this Second Edition aims to collate research that drive the field forward by exploring new concepts, technologies, and applications related to aptamer-based biosensing for POC use. We welcome contributions from researchers across multidisciplinary fields to share new advances, emerging strategies, and future perspectives. Topics of interest include, but are not limited to, the following:

  • Aptamer Selection: new strategies for aptamer discovery, design, modification, and stabilization to enhance binding performance.
  • Biosensor Development: innovative architectures, materials, and fabrication techniques, including wearable technologies, microfluidic platforms, paper-based diagnostics and lab-on-a-chip technologies.
  • Detection Techniques: advances in signal transduction mechanisms such as electrochemical, optical, and mass-sensitive approaches.
  • Translational and Field Applications: demonstrations of aptamer-based biosensors in real-world POC scenarios, including clinical diagnostics, infectious disease management, environmental monitoring, and personalized medicine, highlighting pathways toward practical implementation and commercialization.

We look forward to your valuable contributions to this Second Edition, which will continue to advance the frontier of aptamer-based biosensing and support the development of impactful point-of-care diagnostic solutions.

Prof. Dr. Cecilia Cristea
Dr. Ana Díaz-Fernández
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • aptamer
  • biosensor
  • point-of-care
  • SELEX
  • diagnostics
  • precision medicine

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Published Papers (1 paper)

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Research

14 pages, 2668 KB  
Article
Flexible Cu Nanostructured Laser-Induced Graphene Electrodes for Highly Sensitive and Non-Invasive Lactate Detection in Saliva
by Anju Joshi and Gymama Slaughter
Biosensors 2026, 16(1), 19; https://doi.org/10.3390/bios16010019 - 25 Dec 2025
Cited by 1 | Viewed by 874
Abstract
A scalable and facile fabrication strategy is presented for developing a flexible, nanostructured, non-enzymatic electrochemical sensor for lactate detection based on copper-modified laser-induced graphene (CuNPs/LIG). A one-step electrodeposition process was employed to uniformly decorate the porous LIG framework with copper nanostructures, offering a [...] Read more.
A scalable and facile fabrication strategy is presented for developing a flexible, nanostructured, non-enzymatic electrochemical sensor for lactate detection based on copper-modified laser-induced graphene (CuNPs/LIG). A one-step electrodeposition process was employed to uniformly decorate the porous LIG framework with copper nanostructures, offering a cost-effective and reproducible approach for constructing enzyme-free sensing platforms. Scanning electron microscopy and energy-dispersive X-ray spectroscopy confirmed dense Cu nanostructure loading and efficient interfacial integration across the conductive LIG surface. The resulting CuNPs/LIG electrode exhibited excellent electrocatalytic performance, achieving a sensitivity of 8.56 μA µM−1 cm−2 with a low detection limit of 42.65 μM and a linear response toward lactate concentrations ranging from 100 to 1100 μM in artificial saliva under physiological conditions. The sensor maintained high selectivity in the presence of physiologically relevant interferents. Practical applicability was demonstrated through recovery studies, where recovery rates exceeding 104% showcase the sensor’s analytical reliability in complex biological matrices. Overall, this work establishes a robust, sensitive, and cost-efficient Cu-nanostructured LIG sensing platform, offering strong potential for non-invasive lactate monitoring in real-world biomedical and wearable applications. Full article
(This article belongs to the Special Issue Aptamer-Based Biosensors for Point-of-Care Diagnostics—2nd Edition)
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