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Biosensors
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Applications of Cutting-Edge Biosensors in Environment, Food and Healthcare Field
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Applications of Cutting-Edge Biosensors in Environment, Food and Healthcare Field

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 31 August 2025 | Viewed by 954

Special Issue Editor

Prof. Dr. Ying-Chien Chung

E-Mail Website
Guest Editor
Department of Biological Science and Technology, China University of Science and Technology, Taipei 11581, Taiwan
Interests: environmental biotechnology; applied microbiology; biosensor; biological engineering; bioremediation; microbial fuel cell; waste conversion; environmental engineering with applications in wastewater treatment; wastegas treatment by biofilter; composting technique; the study in chitosan science; photocatalysis technique; the measurement and reduction of greenhouse gases; adsorption technique
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The rapid escalation of global environmental pollution and healthcare demands advanced technologies for environmental, food, and disease monitoring. Molecular and bioelectrochemical techniques enable the precise and comprehensive detection of pollutants, thereby enhancing the effectiveness of environmental surveys, food safety, and health monitoring. In recent years, numerous advanced molecular techniques have been intensively developed, including quantitative PCR, recombinant DNA technology, RNA sequencing, Chromatin immunoprecipitation sequencing, epigenetic modifications, and whole genome sequencing. This Special Issue, entitled “Biosensors for Water, Food, and Environmental Detection”, of the international SCI Journal (2024 IF = 4.9) Biosensors will mainly cover original research which relates to the abovementioned topics, including biosensor materials, biosensors and healthcare, and nano- and micro-technologies biosensors. The Special Issue aims to focus on the recent development of biosensing strategies as applied to environmental survey and monitoring research. Papers selected for this Issue will undergo a peer-review procedure aimed at ensuring the rapid and extensive dissemination of research results. Furthermore, the Special Issue will offer an overview of recent achievements and inspire ideas for current and future research in the field of biosensors. We welcome both original papers and review articles. We eagerly anticipate receiving your exceptional research outcomes.

Prof. Dr. Ying-Chien Chung
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensor
  • food detection
  • toxicity detection
  • tumor markers
  • neuro-biosensors
  • genetic biosensors
  • small RNA biosensor

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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10 pages, 4315 KiB  
Article
Point-of-Care NSE Biosensor for Objective Assessment of Stroke Risk
by Cheng Hsu Chen, Erick Wang, Tsung-Han Lee, Cheng-Chieh Huang, Chun-San Tai, Yan-Ren Lin and Wen-Liang Chen
Biosensors 2025, 15(4), 264; https://doi.org/10.3390/bios15040264 - 20 Apr 2025
Viewed by 112
Abstract
The rapid identification of stroke is critical to improving stroke patient outcomes. Existing protocols for assessing the risk of stroke are subjective and may be further complicated by nonspecific symptoms, increasing the risk of misdiagnosis. Neuron-specific enolase (NSE) has emerged as a promising [...] Read more.
The rapid identification of stroke is critical to improving stroke patient outcomes. Existing protocols for assessing the risk of stroke are subjective and may be further complicated by nonspecific symptoms, increasing the risk of misdiagnosis. Neuron-specific enolase (NSE) has emerged as a promising stroke biomarker. However, current detection methods such as the electrochemiluminescence immunoassay (ECLIA) are time-consuming and costly. In this research, we developed an electrochemical biosensor for the rapid quantification of NSE in whole blood. Mouse stroke models were established, and blood samples collected were analyzed using both hospital-standard ECLIA as well as the biosensor. The biosensor limit of detection was 1.15 ng/mL. NSE measurements were highly correlated between the two methods and were obtained in 5 min using 20 μL of unprocessed whole blood samples. Notably, the biosensor could accurately quantify elevated blood NSE blood that was associated with more severe stroke. Our results demonstrate the utility of the proposed biosensor in pre-hospital settings. Combined with existing stroke assessment methods, the biosensor may enable emergency personnel to identify stroke risk with greater accuracy to optimize the chances of receiving necessary treatment within the effective window. Full article
(This article belongs to the Special Issue Applications of Cutting-Edge Biosensors in Environment, Food and Healthcare Field)
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20 pages, 3343 KiB  
Article
Single-Chamber Microbial Fuel Cell with an Innovative Sensing Component for Real-Time Continual Monitoring of a Wide Range of Cr(VI) Concentrations in Wastewater
by Guey-Horng Wang, Jong-Tar Kuo, Chiu-Yu Cheng and Ying-Chien Chung
Biosensors 2025, 15(3), 158; https://doi.org/10.3390/bios15030158 - 3 Mar 2025
Viewed by 615
Abstract
Hexavalent chromium (Cr(VI)) is toxic, carcinogenic, and harmful to biological systems. Common detection methods, such as colorimetry, atomic absorption spectrometry, ion chromatography, and biological systems, can only be used in the laboratory and do not provide real-time feedback. To address these limitations, the [...] Read more.
Hexavalent chromium (Cr(VI)) is toxic, carcinogenic, and harmful to biological systems. Common detection methods, such as colorimetry, atomic absorption spectrometry, ion chromatography, and biological systems, can only be used in the laboratory and do not provide real-time feedback. To address these limitations, the current study cloned the ChrB gene, which exhibits high specificity in detecting Cr(VI), and the ChrA gene, which exhibits high Cr(VI) tolerance, into Escherichia coli. This recombinant strain, ChrA–ChrB–E. coli, was integrated into a single-chamber microbial fuel cell for accurate continual monitoring over a wide range of Cr(VI) concentrations. ChrA–ChrB–E. coli thrived in temperatures from 25 °C to 45 °C and pH levels between 5 and 8. Its ability to reduce Cr(VI) remained consistent across Cr(VI) forms, carbon sources, and oxyanions. Cyclic voltammetry was employed to verify the electrical activity of the biosensor. The biosensor exhibited a detection limit of 0.0075 mg/L. Under conditions simulating the regulatory emission limit for Cr(VI) of 0.5 mg/L in industrial wastewater, the biosensor achieved a response time of 20 s during continual operation. When tested with synthetic wastewater containing Cr(VI) concentrations from 0.02 to 150 mg/L, the system exhibited high adaptability and facilitated stable monitoring (relative standard deviation ≤ 2.7%). Additionally, the biosensor’s accuracy (−1.73% to 2.5%) matched that of traditional batch methods, highlighting its suitability for real-time Cr(VI) monitoring in aquatic environments. Full article
(This article belongs to the Special Issue Applications of Cutting-Edge Biosensors in Environment, Food and Healthcare Field)
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