Special Issue "Factors affecting in vitro assessment of sperm quality"

A special issue of Biology (ISSN 2079-7737).

Deadline for manuscript submissions: 31 May 2021.

Special Issue Editors

Dr. Miguel A. Silvestre

Guest Editor
Celular Biology, Functional Biology and Physical Antropology; University of Valencia, Valencia (Spain)
Interests: Animal Reproduction; Spermatozoa; Oocyte; in vitro fertilization, artifical insemination
Dr. Jesús L. Yániz

Guest Editor
Institute of Environmental Sciences (IUCA), University of Zaragoza, Huesca (Spain)
Interests: animal reproduction; spermatology; artificial insemination; preservation of animal resources
Dr. Eva Mocé

Guest Editor
Valencian Institute of Agricultural Research (IVIA). Center of Animal Technology (CITA), Segorbe (Spain)
Interests: animal reproduction; andrology; cryobiology; preservation of animal resources
Dr. Carles Soler

Guest Editor
Celular Biology, Functional Biology and Physical Antropology; University of Valencia, Valencia (Spain)
Interests: male reproduction, spermatology, CASA technology development, sperm subpopulaton analysis
Dr. Eduardo Roldán

Guest Editor
Biodiversity and Evolutionary Biology Group, National Museum of Natural Science, Spanish Research Council (CSIC), Madrid, Spain
Interests: reproductive biology, sexual selection, conservation biology, assisted reproduction, determinant of male fertility

Special Issue Information

Dear Colleagues,

The study of the different in vitro sperm parameters is essential to evaluate sperm quality, to predict the fertilizing potential of semen samples and to detect male infertility. These aspects are important in the development of Assisted Reproduction Technologies (IVF, ICSI) in human, the application of artificial insemination in livestock species, the conservation of animal biodiversity and in the study of factors with possible effect on male fertility. Despite the advances in recent years, the predictive ability of in vitro analysis on the fertility potential of semen remains still limited. The combination of different procedures of sperm evaluation, mainly flow cytometry and computer-assisted sperm analysis (CASA), as well as obtaining new knowledge about sperm parameters, could improve this predictive ability. Flow cytometry and CASA systems allow to objectively assess a high number of cells and parameters in a short period of time.  The fact that sperm is the most diverse animal cell in the animal kingdom implies that sperm quality analysis techniques often have to be adapted for each species. This special issue will focus on the most used and advanced in vitro methodologies to evaluate sperm quality for its application in the preservation of seminal doses and the prediction of semen and male fertility in different animal groups, such as mammals, birds, fishes or insects.

Dr. Miguel A. Silvestre
Dr. Jesús L. Yániz
Dr. Eva Mocé
Dr. Carles Soler 
Dr. Eduardo Roldán
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • Spermatozoa
  • In vitro assessment
  • Cytometry
  • Computer-Assisted Sperm Analysis
  • Male fertility
  • Artificial Insemination
  • sperm preservation
  • Assisted Reproductive Technologies
  • Andrology
  • Classification criteria

Published Papers

This special issue is now open for submission.
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