Biomedical Applications of Ionic Liquids and Deep Eutectic Solvents

A special issue of Bioengineering (ISSN 2306-5354).

Deadline for manuscript submissions: closed (31 March 2022) | Viewed by 3664

Special Issue Editors


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Guest Editor
1. John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02138, USA
Interests: gene therapy; bioinspired drug delivery systems; precision medicine; controlled release
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
1. John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02138, USA
Interests: nanomedicine; drug distribution; triggered release; bioinspired drug delivery systems

E-Mail Website
Guest Editor
1. John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02138, USA
Interests: drug delivery; polymeric biomaterials; gene therapy; hemostasis

Special Issue Information

Dear Colleagues,

Biomaterials have become a key component of successful drug delivery, tissue regeneration, and disease diagnostics. While recent developments in smart biomaterials have made major contributions to broadening the current repertoire, there is still an unmet immediate and long-term need for advanced systems with specific functionalities to overcome physicochemical and biological barriers. Ionic liquids and deep eutectic solvents in particular have previously been extensively utilized to facilitate energy storage and gas separation, owing to their notable properties, including thermal stability, conductivity, viscosity, solubility, and antimicrobial effect. Recently, the exploitation of these properties has also spurred their use in pharmaceutical technologies, further leading to investigations in a wide spectrum of biomedical applications.

This Special Issue of Bioengineering on “Biomedical Applications of Ionic Liquids and Deep Eutectic Solvents” will cover original research papers and comprehensive reviews that describe the utilization of ionic liquids/deep eutectic solvents in the development of delivery vehicles for a wide range of therapeutics, including small-molecule drugs, biomacromolecules (peptides, proteins, and nucleic acids), and cells, as well as diagnostic systems. Biomedical applications of interest for this Special Issue include but are not limited to the stabilization of drug formulations, biological transport, controlled release, pharmacokinetics, noninvasive treatment, regenerative medicine, and biosensors.

Dr. Jayoung Kim
Dr. Michael Dunne
Dr. Yongsheng Gao
Guest Editors

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Keywords

  • emulsions
  • controlled release
  • pharmacokinetics
  • nanoparticles
  • polymer composites
  • ionogels
  • patches
  • gene delivery

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Published Papers (1 paper)

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Research

14 pages, 1961 KiB  
Article
Bioengineered System for High Throughput Screening of Kv1 Ion Channel Blockers
by George V. Sharonov, Oksana V. Nekrasova, Ksenia S. Kudryashova, Mikhail P. Kirpichnikov and Alexey V. Feofanov
Bioengineering 2021, 8(11), 187; https://doi.org/10.3390/bioengineering8110187 - 16 Nov 2021
Cited by 2 | Viewed by 3125
Abstract
Screening drug candidates for their affinity and selectivity for a certain binding site is a crucial step in developing targeted therapy. Here, we created a screening assay for receptor binding that can be easily scaled up and automated for the high throughput screening [...] Read more.
Screening drug candidates for their affinity and selectivity for a certain binding site is a crucial step in developing targeted therapy. Here, we created a screening assay for receptor binding that can be easily scaled up and automated for the high throughput screening of Kv channel blockers. It is based on the expression of the KcsA-Kv1 hybrid channel tagged with a fluorescent protein in the E. coli membrane. In order to make this channel accessible for the soluble compounds, E. coli were transformed into spheroplasts by disruption of the cellular peptidoglycan envelope. The assay was evaluated using a hybrid KcsA-Kv1.3 potassium channel tagged with a red fluorescent protein (TagRFP). The binding of Kv1.3 channel blockers was measured by flow cytometry either by using their fluorescent conjugates or by determining the ability of unconjugated compounds to displace fluorescently labeled blockers with a known affinity. A fraction of the occupied receptor was calculated with a dedicated pipeline available as a Jupyter notebook. Measured binding constants for agitoxin-2, charybdotoxin and kaliotoxin were in firm agreement with the earlier published data. By using a mid-range flow cytometer with manual sample handling, we measured and analyzed up to ten titration curves (eight data points each) in one day. Finally, we considered possibilities for multiplexing, scaling and automation of the assay. Full article
(This article belongs to the Special Issue Biomedical Applications of Ionic Liquids and Deep Eutectic Solvents)
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