Advances in Structural Biology Methods

Dear Colleagues,

Advances in structural biology methods rely on the development of analytical procedures. This Special Issue invites articles on:

• Complementary techniques that have revolutionalised the study of protein structure and dynamics, such as “hydrogen-deuterium exchange” combined with NMR spectroscopy or mass spectrometry, which have made possible to visualise kinetics of protein folding at amino acid resolution; tryptophan fluorescence spectroscopy that has enabled capturing local structural changes and dynamics of proteins on fast time scales (e.g. picoseconds); X-ray crystallography as an invaluable tool that continues to contribute greatly to protein structure determination;
• Pioneering methods of Protein Footprinting coupled with mass spectrometry, also referred to as "Radical Probe Mass Spectrometry (RPMS)", or “Synchrotron X-Ray Radiolysis”, which enable the study of three-dimensional protein structures, folding and macromolecular interactions dynamics on fast timescales (down to sub-milliseconds); including reviews of this fast growing technology, covering advances of hydroxyl radical production, updates in capabilities of Synchrotron Facilities, and a range of biological and pharmaceutical applications of protein footprinting;
• Historical perspectives and emerging techniques applied to the study of protein structure and dynamics.

This Special Issue initiative invites articles with an overview of this fast-growing technology, covering advances of hydroxyl radical production, updates in capabilities of synchrotron facilities, and a range of biological and pharmaceutical applications of protein footprinting.

Dr. Simin D. Maleknia
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.