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A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Nanotechnology and Applied Nanosciences".

Deadline for manuscript submissions: closed (31 October 2019) | Viewed by 2885

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Department of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, 20133 Milan, Italy
Interests: essential oils; bioactive phytochemicals; ethnopharmacology; antimicrobial resistance; one health; food security
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Special Issue Information

Dear Colleagues,

Directive 2009/128/EC establishes the sustainable use of pesticides by reducing the risks and impacts of pesticide use on human health and the environment and promoting the use of integrated pest management, and alternative approaches or techniques such as non-chemical alternatives to pesticides. In addition, these measures are complementary to Regulation (EC) No 396/2005, which declares that a high level of consumer protection needs to be ensured, with provisions relating to maximum levels of pesticide residues in food and feed of plant and animal origin. Pesticide residues include active substances, metabolites, and/or breakdown or reaction products of active substances currently or formerly used in plant protection products. Accordingly, maximum residue level (MRL) refers to the upper legal level of a concentration for a pesticide residue in food or feed set by the Codex Alimentarius Commission and depending on good agricultural practice, and to the lowest consumer exposure necessary to protect vulnerable consumers. Of course, these issues are relevant worldwide, and not only in a European context, and every country adopts its own legislation.

In this very wide context, we invite investigators to submit both original research and review articles that explore all these aspects. Potential topics include but are not limited to innovative natural products potentially exploited in crop protection, including their nanoformulations.

Dr. Sara Sara Vitalini
Prof. Dr. Marcello Iriti
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • • Biopesticides
  • • Biomaterials
  • • Nanotechnologies
  • • Nanomaterials
  • • Nanostructured materials
  • • Nanoformulates
  • • Food safety
  • • Nanotoxicology.

Published Papers (1 paper)

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12 pages, 881 KiB  
Elicitation of the Allelopathic Potential of Rice by Methyl Salicylate Treatment
by Babita Patni, Sudhir Kumar Guru, Marcello Iriti and Sara Vitalini
Appl. Sci. 2019, 9(22), 4881; - 14 Nov 2019
Cited by 4 | Viewed by 2140
Weed infestation is one of the most severe problems affecting rice production worldwide. Current weed control practices are either costly or pose a threat to the environment. The use of competitive rice genotypes seems to be a promising strategy. The aim of our [...] Read more.
Weed infestation is one of the most severe problems affecting rice production worldwide. Current weed control practices are either costly or pose a threat to the environment. The use of competitive rice genotypes seems to be a promising strategy. The aim of our study was to improve the allelopathic potential of three selected rice genotypes by foliar application of methyl salicylate (MeSA), a signaling molecule eliciting the plant defense response. Aqueous extracts of the shoots and roots of two competitive (UPR 2962-6-2-1 and Govind) and one non-competitive (UPR 2992-17-3-1) rice genotypes treated with MeSA solution at different concentrations (1 mM, 2 mM, and 3 mM) showed phytotoxic effects on the growth of the weed Echinochloa colona. Specifically, shoot and root extracts obtained from the competitive rice genotypes similarly decreased both the E. colona germination rate (9% to 44.5%) and the length of its roots and shoots (11% to 48%). Extracts of the non-competitive rice genotype showed a similar trend but lower effects, inhibiting the E. colona germination up to 32% and reducing the growth of the weed roots and shoots by 6–23.5% and 7–28%, respectively, according to the increasing MeSA concentrations. High-performance liquid chromatography (HPLC) analysis demonstrated an increase in different allelopathic phenolic acids in the three rice genotypes in response to MeSA treatments. Among the detected compounds, vanillic and protocatechuic acids showed the greatest differences compared to controls with values up to 2.1-fold higher in shoots of the two competitive UPR-2962-6-2-1 and Govind rice genotypes, while their roots were characterized by the greatest increases of 8-hydroxyquinoline (8.2-fold) and protocatechuic acid (1.7-fold). Differently, non-competitive genotype UPR 2992-17-3-1 showed the highest increase for gallic acid (2.2-fold) in shoots and for p-hydroxybenzoic acid (2.4-fold) in roots. Lastly, MeSA was found to improve the competitiveness of rice genotypes without any detrimental effect on the host plant. Full article
(This article belongs to the Special Issue (Nano)bioagrochemicals)
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