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Green Chemistry and Application of Biorefineries

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Energy Science and Technology".

Deadline for manuscript submissions: 1 November 2025 | Viewed by 312

Special Issue Editors


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Guest Editor
Biotransformation and Organic Biocatalysis Research Group, Department of Exact Sciences, Santa Cruz State University, Ilhéus 45654-370, Brazil
Interests: resource recovery; microalgae; nature-based solutions; exopolysaccharides; bioenergy; wastewater; cyanobacteria

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Guest Editor
REQUIMTE/LAQV, ISEP, Polytechnic of Porto, Rua Dr. António Bernardino de Almeida, 4249-015 Porto, Portugal
Interests: (bio)sensors; application of functional nanostructured materials; green technologies; new methodologies for (electro)analysis; environmental chemistry; monitoring/biomonitoring
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Guest Editor Assistant
Biorefinery Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico
Interests: biotechnlogy; high added-value producs; lignocellulosic biorefinery; biorefineries and bio-based production; bioprocess enginieering; agro-industrial wastes; conceptual design

Special Issue Information

Dear Colleagues,

As the global population continues to grow, the demand for food, water, energy and other resources is expected to rise, leading to an increased generation of vast amounts of lignocellulosic and agri-food by-products. These by-products are often referred to as "waste" when the processes of circular economy practices are not implemented. Recovering valuable metabolites or transforming these materials into useful compounds or products in a sustainable manner has now become a necessity. Our society faces pressing challenges, including resource scarcity, environmental pollution, and climate change. The adoption of circular economy approaches enable sustainable energy solutions, maximizes the extraction of high-value compounds from industrial raw materials, and reduces overall resource consumption. In particular, lignocellulosic biomass and other agri-food by-products serve as valuable raw materials for developing green chemistry products and processes. These include bio-based chemicals and compounds such as antioxidants, vitamins, antimicrobials, biostimulants for agriculture, reducing agents for metal nanoparticle production, catalytic agents for biopolymer processing, structural and adsorptive materials, as well as sources of sugars, and other macro- and micronutrient applications. Such bioprocesses, involving fungi, bacteria, yeasts, and microalgae, contribute to the development of high-value industrial compounds within an integrated biorefinery framework.

We are pleased to invite you to submit your research and contribute to this Special Issue on Green Chemistry and Application of Biorefineries, which aims to explore both practical and theoretical aspects of utilizing lignocellulosic and agri-food by-products, often mistakenly classified as waste, to generate valuable industrial green chemistry products. The focus is on mitigating waste generation, optimizing resource utilization, and reducing greenhouse gas emissions, all while integrating these processes into biorefinery concepts. 

We welcome innovative approaches that fully utilize lignocellulosic and agri-food by-products within circular economy strategies. Contributions may explore their integration into existing biorefineries or propose new theoretical biorefinery concepts tailored for filamentous fungi, yeasts, bacteria, and microalgae-based bioprocesses. Additionally, this Special Issue targets biomass derived from green chemistry and biological processes, aiming to develop technologies for the complete utilization and cyclical valorization of by-products as inputs or target compounds. We invite original research articles and reviews in (but not limited to) the following areas: 

  1. Utilization of lignocellulosic and agri-food biomass for extracting high-added value compounds;
  2. Application of extracted compounds in green chemistry;
  3. Novel methods and approaches for lignocellulosic and agri-food biomass processing;
  4. Solid-state and submerged fermentation for industrial metabolite and biomass production;
  5. Microalgae-based processes for valorizing lignocellulosic and agri-food by-products;
  6. Development of adsorptive and structural materials from biomass;
  7. Green catalysis using lignocellulosic and agri-food biomass;
  8. Integration of industrial wastewater into biomass valorization for green chemistry applications;
  9. New biorefinery concepts for the cyclical and full valorization of by-products, from input materials to final products;
  10. Lignocellulosic-based polymers and waste minimization.

We look forward to receiving your contributions.

Dr. Igor Sampaio
Prof. Dr. Simone Morais
Guest Editors

Dr. Karla González-Gloria
Guest Editor Assistant

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • agri-food by-products
  • circular economy
  • bioprocessing
  • fermentation
  • microalgae cultivation
  • bio-based materials
  • sustainable processes

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Published Papers (1 paper)

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Research

18 pages, 1283 KiB  
Article
Chromochloris zofingiensis-Based Treatment of Whey Wastewater for Biorefinery Application: Biomass, Nutrient Removal, Astaxanthin and Lipid Production
by Houria El Bakraoui, Amina Malaki, Miloudia Slaoui and Céline Laroche
Appl. Sci. 2025, 15(11), 5832; https://doi.org/10.3390/app15115832 - 22 May 2025
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Abstract
The dairy industry generates substantial quantities of wastewater, primarily whey wastewater, posing environmental challenges. Current treatment methods involve physical, chemical, and biological processes, but efficient solutions are still sought. Biological treatments using microalgae are gaining attention due to their potential to remove pollutants [...] Read more.
The dairy industry generates substantial quantities of wastewater, primarily whey wastewater, posing environmental challenges. Current treatment methods involve physical, chemical, and biological processes, but efficient solutions are still sought. Biological treatments using microalgae are gaining attention due to their potential to remove pollutants from wastewater and generate valuable products, making them an alternative way to improve environmental sustainability. The physicochemical characterization of whey effluents reveals a high organic content, an acidic pH, and elevated nutrient levels. This study investigates the potential of Chromochloris zofingiensis (formerly known as Chlorella zofingiensis) for treating whey wastewater using three concentrations, 10%, 20%, and 50%, over a 7-day culture period. The optimal concentration of whey wastewater for biomass, nutrient removal, astaxanthin, and lipid production was found to be 10%. At this concentration, C. zofingiensis achieved a biomass of 3.86 g L−1 and a removal efficiency of nutrients between 77.08% and 99.90%. Analysis of pigment production revealed decreases in chlorophyll and carotenoid production with increasing whey wastewater concentration, while lipid and astaxanthin production peaked at the 10% dilution. The chlorophyll a, chlorophyll b, total carotenoid, astaxanthin, and lipid contents were, respectively, 11.49 mg g−1, 4.56 mg g−1, 4.04 mg g−1, 0.71 mg g−1, and 30.49% in 10% whey wastewater. The fatty acid profiles indicated the predominance of saturated and unsaturated fatty acids, enhancing the biofuel potential of C. zofingiensis cultivated in whey wastewater. These findings demonstrate the dual benefit of using C. zofingiensis for sustainable whey wastewater treatment and high-value bioproduct generation, supporting the development of circular biorefinery systems. Full article
(This article belongs to the Special Issue Green Chemistry and Application of Biorefineries)
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