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Biotechnology in Animals

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (31 December 2025) | Viewed by 3036

Special Issue Editors


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Guest Editor
Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia
Interests: pig breeding; animal biotechnology; genomics; animal science; meat quality

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Guest Editor
Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia
Interests: animal product quality; meat science; authentication of meat and meat products; nutrigenomics
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Division of Animal Science, Faculty of Agriculture, University of Zagreb, Svetošimunska Cesta 25, 10000 Zagreb, Croatia
Interests: pig breeding; pig reproduction; selection; breeding value estimation; breeding programmes; genetic diversity; bionformatics

Special Issue Information

Dear Colleagues,

Animal biotechnology is a specialised branch of biotechnology that uses advanced molecular biology techniques to genetically modify and manipulate the genome of animals with the aim of improving or introducing specific traits. These modifications increase the usefulness of animals in various fields, e.g., in pharmaceuticals, where genetically modified animals can produce therapeutic proteins and drugs; in agriculture, where traits such as faster growth, disease resistance, and higher productivity are emphasised; and in industry, where animals can be modified to produce valuable biological substances or support sustainable practices. This field has developed rapidly in recent decades and thus plays a crucial role in addressing global challenges in the areas of food security, medicine, and environmental protection, offering ground-breaking solutions in areas such as medicine, agriculture, and environmental sustainability. It encompasses a wide range of technologies, from genetic modification to cloning and reproductive technologies, all aimed at improving the productivity, health, and benefits of animals for humans. In agriculture, this has led to improved feed efficiency and disease resistance in livestock. Applications include the creation of genetically modified livestock with better growth rates or disease resistance and the use of animals as bioreactors to produce therapeutic proteins. Animal biotechnology has made a significant contribution to the pharmaceutical industry, particularly through the production of therapeutic proteins and drugs. Cloning and reproductive technologies such as in vitro fertilisation (IVF) are also part of this field and help to accelerate selective breeding. Possible topics include but are not limited to the following:

  • Biotechnology;
  • Livestock;
  • Genetic improvement of livestock;
  • Genetically modified animals;
  • Gene editing;
  • Comparative genomics and biodiversity;
  • Reproductive biotechnologies;
  • Disease resistance;
  • Therapeutic proteins.

Dr. Kristina Gvozdanović
Dr. Ivona Djurkin Kušec
Dr. Dubravko Škorput
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biotechnology
  • livestock
  • genetic improvement of livestock
  • genetically modified animals
  • gene editing
  • comparative genomics and biodiversity
  • reproductive biotechnologies
  • disease resistance
  • therapeutic proteins

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Published Papers (2 papers)

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Research

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23 pages, 1067 KB  
Article
Nutritional Efficiency of Mulberry Leaves Enriched with a Concentrated Probiotic in Two Breeds (Kahuri/T and AO/T) of Bombyx mori L. Silkworm
by Mihaela Hăbeanu, Anca Gheorghe, Nicoleta Aurelia Lefter and Teodor Mihalcea
Appl. Sci. 2026, 16(2), 961; https://doi.org/10.3390/app16020961 - 16 Jan 2026
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Abstract
This study aimed to assess the effect of mulberry leaf fortification with a probiotic (Enterococcus faecium and rosemary) on larval development, feed utilization efficiency, digestive performance, and cocoon-related traits in two Bombyx mori breeds. The results showed that a probiotic addition to [...] Read more.
This study aimed to assess the effect of mulberry leaf fortification with a probiotic (Enterococcus faecium and rosemary) on larval development, feed utilization efficiency, digestive performance, and cocoon-related traits in two Bombyx mori breeds. The results showed that a probiotic addition to the silkworms’ diet, particularly at a 2% inclusion level, enhances key productive traits such as larval weight, silk gland weight, cocoon weight, digestibility, and feed efficiency, while reducing excreta. Breed-specific differences were evident, with Kahuri/T exhibiting superior nutrient intake, assimilation, and overall productivity compared to AO/T (p < 0.0001). Productive traits during the 5th instar increased significantly over time, with both larval growth and silk gland development following rapid, exponential patterns. The Kahuri/T breed and the probiotic-supplemented groups—particularly at a 2% concentration—showed the most notable improvements compared with the AO/T breed and the control diet. Pearson correlation analyses identified strong positive relationships between feed efficiency, protein intake, and silk yield, underscoring probiotics as a viable strategy for sustainable productivity gains in sericulture. In conclusion, mulberry leaf fortification with Enterococcus faecium and rosemary, especially at a 2% inclusion level, significantly improved performance and nutrient utilization, with the strongest responses observed in the Kahuri/T breed. These findings highlight probiotic supplementation as an effective and sustainable strategy for enhancing sericulture. Full article
(This article belongs to the Special Issue Biotechnology in Animals)
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Review

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18 pages, 295 KB  
Review
A Comprehensive Review: Molecular and Genealogical Methods for Preserving the Genetic Diversity of Pigs
by Vladimir Margeta, Dubravko Škorput, Ivona Djurkin Kušec, Zlata Kralik, Goran Kušec and Kristina Gvozdanović
Appl. Sci. 2025, 15(6), 3394; https://doi.org/10.3390/app15063394 - 20 Mar 2025
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Abstract
Anthropogenic activities and rapidly increasing climate change have led to a significant loss of genetic diversity in domestic animals. Genealogical data have traditionally been used to monitor genetic diversity. However, due to dependency on pedigree completeness and significant errors that can occur in [...] Read more.
Anthropogenic activities and rapidly increasing climate change have led to a significant loss of genetic diversity in domestic animals. Genealogical data have traditionally been used to monitor genetic diversity. However, due to dependency on pedigree completeness and significant errors that can occur in genealogical records, inaccurate estimation of population parameters, such as the inbreeding coefficient or effective population size, might occur. To reduce possible errors, it is necessary to combine genealogical data with molecular data. An integrated approach using genealogical and molecular data leads to the optimization of breeding programs while controlling the inbreeding that can occur within the population of domestic animals. Molecular techniques such as single nucleotide polymorphism (SNP) genotyping, whole-genome sequencing (WGS), or genome-wide association studies (GWASs) enable a detailed understanding of breed-specific genetic profiles and their use in conservation programs. In addition, molecular methods such as structural variation (SV) analysis and pangenome and epigenomic profiling provide a more comprehensive insight into genetic diversity. The conservation of genetic diversity is of particular importance for an autochthonous domestic breed due to its resilience to unfavorable climatic conditions, its specific productive traits, and its disease resistance. A combined approach of genealogical and molecular data helps to maintain genetic diversity and sustainable agricultural systems under evolving environmental challenges. Full article
(This article belongs to the Special Issue Biotechnology in Animals)
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