Recent Trends towards Genetics-Based Methods for Pest Management in Agroecosystems—Second Edition

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Pest and Disease Management".

Deadline for manuscript submissions: closed (30 November 2024) | Viewed by 1309

Special Issue Editors


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Guest Editor
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
Interests: plant-insect relationship; plant resistant to pests; environmental entomology and climate change biology; insect physiology and insect ecology; ecological control of pests
Special Issues, Collections and Topics in MDPI journals
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Interests: new methods or techniques for pest management; integrated pest management
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The second edition of our Special Issue, titled "Recent Trends towards Genetics-Based Methods for Pest Management in Agroecosystems—Second Edition", is an extension of our first edition, providing space for contributions related to this important branch of pest control. The primary aim of this Special Issue is to provide a platform for the presentation and discussion of the most recent trends in genetic methods for pest control, emphasizing their new applications and potential contributions to integrated pest management (IPM) in the agricultural field.

In the face of the challenges posed by insect pests and the shortcomings of conventional control methods, this edition seeks to shed light on innovative approaches that leverage genetic principles for more effective pest management in agroecosystems. By welcoming and encouraging scientists who are engaged in researching these issues, we hope to gather a collection that contributes to the advancement of genetic methods for pest control. Researchers are invited to share their latest research results to collectively address the evolving landscape of genetic pest management in agroecosystems.

Prof. Dr. Fajun Chen
Dr. Lanzhi Han
Guest Editors

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Keywords

  • insect-resistant genetically modified crops
  • RNAi and CRISPR/Cas9 editing methods
  • traditional genetic breeding techniques
  • diverse applications of insect-resistant crops
  • genetically sterile techniques for insect pests
  • transgenic insects
  • biopesticides

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Published Papers (1 paper)

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Research

12 pages, 2206 KiB  
Article
Deletion of the 3′ End of the Introduced cry1Ac Gene Retains the Insecticidal Activity in Transgenic Cotton
by Chunmeng Huang, Yongjun Zhang, Huilin Yu, Chenhui Hou, Haoyuan Guan, Xiuping Chen and Jiajian Xie
Agronomy 2024, 14(7), 1400; https://doi.org/10.3390/agronomy14071400 - 27 Jun 2024
Viewed by 819
Abstract
The structure and expression of exogenous genes in transgenic crops are critical for the target traits. R7569 has the same exogenous insertion structure as the transgenic insect-resistant cotton MON531 but with a deletion in the 3′ end of the cry1Ac gene and the [...] Read more.
The structure and expression of exogenous genes in transgenic crops are critical for the target traits. R7569 has the same exogenous insertion structure as the transgenic insect-resistant cotton MON531 but with a deletion in the 3′ end of the cry1Ac gene and the terminator region. Thus, in the present study, transcription, expression, and insecticidal activity assays were conducted to determine the function of the truncated cry1Ac gene. R7569 has a truncated cry1Ac gene with a length of 2554 bp encoding 881 amino acids, and the transcription termination site was mainly concentrated downstream of the truncated position and extended 160–270 bp from the truncated position using rapid amplification of cDNA ends (RACE). The transcript levels of the cry1Ac gene in R7569 were significantly higher than those of MON531 implants, except for during the boll stage. The content of the Cry1Ac protein in R7569 was higher than that of MON531 in the cotton leaf in all three periods. The corrected mortality rates of R7569 and MON531 against bollworms were 93.09% and 88.83%, respectively. The LC50 value of R7569 was 0.732 ng/g (dw), indicating a high level of resistance to bollworm. In this study, for the first time, we found a partial deletion of the target gene in commercially applied transgenic crops, and the partial deletion of the 3′ end of the cry1Ac gene retained a better transcription, expression level, and insecticidal activity, which can provide a specific case for the safety evaluation of transgenic crops. Full article
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