COVID, Volume 4, Issue 9
September 2024 - 11 articles
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Cover Story: Human coronavirus 229E causes the rearrangement of host intracellular membranes to form replication organelles through hijacking members of the ESCRT pathway. We found that the ESCRT protein VPS4A was required for the formation of replication organelles and the localization of viral RNA to these structures. Mutation and inhibition of VPS4A significantly reduced virus replication and prevented the formation of replication organelles, exposing the viral RNA and triggering an inflammatory immune response. A similar phenomenon was observed for the OC43 coronavirus, indicating conservation across coronavirus genera. Furthermore, our data suggest that ESCRTs CHMP4B and VPS37A are required for virus replication and post-replication stages, respectively, highlighting the importance of ESCRTs in coronavirus replication. View this paper