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Methods and Protocols, Volume 7, Issue 5

October 2024 - 19 articles

Cover Story: This protocol presents a robust method for extracting intra- and extracellular metabolites from gut bacterial cultures. The co-culture of bacterial populations is vital for studying cell interactions, cross-feeding, and metabolic interactions in complex microbial environments. The protocol provides a reproducible, physiologically relevant method for culturing under controlled environmental conditions and extracting metabolites without quenching, cell disruption, freeze–thaw cycles, or sonication, preserving metabolite integrity by utilizing fast vacuum filtration and centrifugation. The extraction method is efficient, taking just 4 hours to complete. View this paper
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Articles (19)

  • Article
  • Open Access
1,752 Views
12 Pages

Activity Identification, Classification, and Representation of Wheelchair Sport Court Tasks: A Method Proposal

  • Mathieu Deves,
  • Christophe Sauret,
  • Ilona Alberca,
  • Lorian Honnorat,
  • Yoann Poulet,
  • Arnaud Hays and
  • Arnaud Faupin

18 October 2024

Background: Monitoring player mobility in wheelchair sports is crucial for helping coaches understand activity dynamics and optimize training programs. However, the lack of data from monitoring tools, combined with a lack of standardized processing a...

  • Protocol
  • Open Access
1,697 Views
8 Pages

Validity and Reliability According to the Type of Examiners in the Process of Calibrating Dental Caries Experience Using the DMFT Index

  • Anna Paola Fernández-Coll,
  • María Claudia Garcés-Elías,
  • Jorge A. Beltrán,
  • Roberto A. León-Manco and
  • Janett Mas-López

16 October 2024

The process of examiner calibration is an essential step in all epidemiological research, as it aims to ensure uniform interpretation, understanding, and application of the instrument to be used. This ensures that the data collected will be valid and...

  • Protocol
  • Open Access
1 Citations
2,125 Views
12 Pages

10 October 2024

Polyphenols are responsible for wine colour and astringency, and, as antioxidants, they also have beneficial health properties. In this work, we developed a robust full-scan high-resolution mass spectrometry method for the quantification of 90 phenol...

  • Study Protocol
  • Open Access
1,894 Views
12 Pages

Maintaining and improving the health and well-being of older people in rural communities through integrated care is essential to address this cohort’s frailty risk. The Indigo 4Ms Tool for health workers is a rural-specific approach to providin...

  • Study Protocol
  • Open Access
2 Citations
8,555 Views
9 Pages

Effects of Sucralose Supplementation on Glycemic Response, Appetite, and Gut Microbiota in Subjects with Overweight or Obesity: A Randomized Crossover Study Protocol

  • Zeniff Reyes-López,
  • Viridiana Olvera-Hernández,
  • Meztli Ramos-García,
  • José D. Méndez,
  • Crystell G. Guzmán-Priego,
  • Miriam C. Martínez-López,
  • Carlos García-Vázquez,
  • Carina S. Alvarez-Villagomez,
  • Isela E. Juárez-Rojop and
  • Juan C. Díaz-Zagoya
  • + 1 author

Sucralose stands as the most common non-nutritive sweetener; however, its metabolic effects have sparked significant controversy over the years. We aim to examine the effects of sucralose daily intake on glycemia, subjective appetite, and gut microbi...

  • Study Protocol
  • Open Access
1 Citations
1,933 Views
8 Pages

Prophylactic Administration of Perampanel for Post-Stroke Epilepsy (PROPELLER Study): A Trial Protocol

  • Shuichi Yamada,
  • Ichiro Nakagawa,
  • Masashi Kotsugi,
  • Kiyoshi Asada and
  • Masato Kasahara

Background: Post-stroke epilepsy can reduce patients’ abilities to carry out various activities of daily living. Despite their importance in preventing the onset of post-stroke epilepsy, the prophylactic administration of antiepileptic drugs is...

  • Study Protocol
  • Open Access
3 Citations
2,219 Views
10 Pages

Effect of Telenursing on Supportive Care Needs in Patients with Melanoma and Lung Cancer on Targeted Therapies: A Randomised Controlled Trial Study Protocol

  • Aurora De Leo,
  • Gloria Liquori,
  • Alessandro Spano,
  • Nicolò Panattoni,
  • Sara Dionisi,
  • Laura Iacorossi,
  • Noemi Giannetta,
  • Irene Terrenato,
  • Emanuele Di Simone and
  • Marco Di Muzio
  • + 1 author

Background: Telenursing comprises a set of tools and interventions enabling nurses to provide remote care. This study aims to assess the impact of telenursing interventions on the supportive care needs of patients with melanoma and lung cancer who ar...

  • Study Protocol
  • Open Access
2,175 Views
16 Pages

29 September 2024

Although South Africa was the first country to register and roll out oral pre-exposure prophylaxis (PrEP) biomedical human immunodeficiency virus (HIV) prevention intervention in sub-Saharan Africa (SSA), its uptake remains low, particularly among ad...

  • Protocol
  • Open Access
2 Citations
2,793 Views
11 Pages

Use of Decellularized Bio-Scaffolds for the Generation of a Porcine Artificial Intestine

  • Sharon Arcuri,
  • Georgia Pennarossa,
  • Madhusha Prasadani,
  • Fulvio Gandolfi and
  • Tiziana A. L. Brevini

27 September 2024

In recent years, great interest has been focused on the development of highly reproducible 3D in vitro models that are able to mimic the physiological architecture and functionality of native tissues. To date, a wide range of techniques have been pro...

  • Protocol
  • Open Access
2 Citations
1,818 Views
13 Pages

26 September 2024

Mechanical signals from the extracellular matrix are crucial in guiding cellular behavior. Two-dimensional hydrogel substrates for cell cultures serve as exceptional tools for mechanobiology studies because they mimic the biomechanical and adhesive c...

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Methods Protoc. - ISSN 2409-9279