Table of Contents
Methods Protoc., Volume 1, Issue 3 (September 2018)
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Cover Story (view full-size image) Generation of locus-specific genomic editing using CRISPR/Cas9 in cell lines and primary cells is [...] Read more. Generation of locus-specific genomic editing using CRISPR/Cas9 in cell lines and primary cells is becoming routine lab practice. However, identification of specifically induced mutations and sequence changes can require laborious screening of hundreds of clones. This protocol permits high-throughput genotyping of edited cells using next generation sequencing. In addition to allowing for the screening of hundreds of clones simultaneously, this approach provides the edited sequence of each allele and can be applied to multiple loci in a single sequencing run. View this paper.