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Methods Protoc. 2018, 1(3), 33; https://doi.org/10.3390/mps1030033

Improved Detection and Fragmentation of Disulphide-Linked Peptides

1
Food & Bio-Based Products, AgResearch Ltd., 1365 Springs Road, Lincoln 7674, New Zealand
2
Biomolecular Interaction Centre, University of Canterbury, Christchurch 8140, New Zealand
3
Riddet Institute, Massey University, Palmerston North 4442, New Zealand
4
Wine, Food & Molecular Biosciences, Lincoln University, Lincoln 7674, New Zealand
*
Author to whom correspondence should be addressed.
Received: 6 August 2018 / Revised: 24 August 2018 / Accepted: 28 August 2018 / Published: 3 September 2018
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Abstract

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required. View Full-Text
Keywords: disulphide bonds; MALDI-TOF/TOF MS; aniline; proof-of-existence disulphide bonds; MALDI-TOF/TOF MS; aniline; proof-of-existence
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Maes, E.; Clerens, S.; Dyer, J.M.; Deb-Choudhury, S. Improved Detection and Fragmentation of Disulphide-Linked Peptides. Methods Protoc. 2018, 1, 33.

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