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Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots

1
Banco de Germoplasma ISOPlexis, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal
2
Departamento de Biologia, Pólo da Mitra Apartado 94, 7002-554 Évora, Portugal
3
Instituto de Ciências da Terra (ICT), Colégio Luís António Verney, Rua Romão Ramalho 59, 7000-671 Évora, Portugal
*
Author to whom correspondence should be addressed.
Methods Protoc. 2018, 1(3), 34; https://doi.org/10.3390/mps1030034
Received: 6 July 2018 / Revised: 17 August 2018 / Accepted: 29 August 2018 / Published: 5 September 2018
Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots. View Full-Text
Keywords: ectomycorrhiza; mycorrhiza-like structures; stone pine; adventitious roots; Hartig net ectomycorrhiza; mycorrhiza-like structures; stone pine; adventitious roots; Hartig net
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MDPI and ACS Style

Ragonezi, C.; Zavattieri, M.A. Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots. Methods Protoc. 2018, 1, 34. https://doi.org/10.3390/mps1030034

AMA Style

Ragonezi C, Zavattieri MA. Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots. Methods and Protocols. 2018; 1(3):34. https://doi.org/10.3390/mps1030034

Chicago/Turabian Style

Ragonezi, Carla, and Maria A. Zavattieri. 2018. "Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots" Methods and Protocols 1, no. 3: 34. https://doi.org/10.3390/mps1030034

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