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Methods Protoc. 2018, 1(3), 34; https://doi.org/10.3390/mps1030034

Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots

1
Banco de Germoplasma ISOPlexis, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal
2
Departamento de Biologia, Pólo da Mitra Apartado 94, 7002-554 Évora, Portugal
3
Instituto de Ciências da Terra (ICT), Colégio Luís António Verney, Rua Romão Ramalho 59, 7000-671 Évora, Portugal
*
Author to whom correspondence should be addressed.
Received: 6 July 2018 / Revised: 17 August 2018 / Accepted: 29 August 2018 / Published: 5 September 2018
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Abstract

Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots. View Full-Text
Keywords: ectomycorrhiza; mycorrhiza-like structures; stone pine; adventitious roots; Hartig net ectomycorrhiza; mycorrhiza-like structures; stone pine; adventitious roots; Hartig net
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ragonezi, C.; Zavattieri, M.A. Histological Studies of Mycorrhized Roots and Mycorrhizal-Like-Structures in Pine Roots. Methods Protoc. 2018, 1, 34.

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