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Effect of Casein Hydrolysates on Intestinal Cell Migration and Their Peptide Profiles by LC-ESI/MS/MS

1
Department of Food Science, Aarhus University, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark
2
Department of Animal Science, Aarhus University, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark
*
Author to whom correspondence should be addressed.
Foods 2019, 8(3), 91; https://doi.org/10.3390/foods8030091
Received: 27 January 2019 / Revised: 27 February 2019 / Accepted: 2 March 2019 / Published: 6 March 2019
(This article belongs to the Section Food Nutrition)
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Abstract

Potential beneficial effects of bioactive peptides derived from casein on epithelial cellular wound healing in the gastrointestinal tract were studied. Bovine casein was digested by a combination of pepsin and pancreatic proteases at different time intervals to represent ranges of duration of gastrointestinal digestion. Intestinal epithelial cells were used as an in vitro model of the small intestine. The effect of casein hydrolysates on cell migration was studied by scratch assay as a model of wound healing. Casein digested by pepsin and pancreatin for 10 to 30 min were found to have a significant stimulatory effect of >40% on cell migration relative to the control. A potential effect of casein gastrointestinal digests on gastro-intestinal wound healing has not previously been reported. The peptide profiles of active as well as inactive casein hydrolysates were characterised by liquid chromatography coupled to ion trap tandem mass spectrometry. By comparison of identified peptides in active and inactive casein hydrolysates, a pool of 11 peptides derived from casein were identified as potential candidates for effects on cell migration. Searching the milk bioactive peptide database (MBPDB) showed that 15 of the identified peptides had known biological functions such as antimicrobial, antioxidant, and immunomodulatory activity. View Full-Text
Keywords: dairy; wound healing; IEC-6 cells; scratch assay; functional foods; bioactive; peptide dairy; wound healing; IEC-6 cells; scratch assay; functional foods; bioactive; peptide
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Nielsen, S.D.; Purup, S.; Larsen, L.B. Effect of Casein Hydrolysates on Intestinal Cell Migration and Their Peptide Profiles by LC-ESI/MS/MS. Foods 2019, 8, 91.

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