Nucleic acid amplification (NAA) is a technique that increases the number of copies of a gene, making it possible to detect microorganisms. This technique is often used in clinical tests, biochemical analysis, and environmental assays, to mention only a few. However, developing portable,
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Nucleic acid amplification (NAA) is a technique that increases the number of copies of a gene, making it possible to detect microorganisms. This technique is often used in clinical tests, biochemical analysis, and environmental assays, to mention only a few. However, developing portable, robust, and low-cost measurement platforms to evaluate NAA products remains a technological challenge. Therefore, in this work, we introduce an attractive unit for detecting and quantifying nucleic acids based on the capacitively coupled contactless conductivity detection (
D) principle. The proposed unit, Re
D, combines electrical resonance with
D to enhance sensitivity when evaluating an NAA reaction. The Re
D units advantages are twofold: (i) the transducer is electrically isolated to allow its reuse, and (ii) the induced electrical resonance in the Re
D unit minimizes the stray capacitances of the conventional
D assays, which enhances sensitivity, increases the linear operating range, and improves the limit of detection (LoD). Furthermore, we evaluated the proposed device for quantifying different concentrations of SARS-CoV-2 genetic material and compared it with measurements from a conventional
D unit. Thus, we demonstrate that the Re
D unit can measure concentrations of NAA products with an LoD of 0.24
and a sensitivity of 5.618
. These results position the Re
D unit close to the state-of-the-art NAA testing platforms, with the added value of a low cost, robustness, reusability, and affordability.
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