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Volume 72
Issue 2
10.3797/scipharm.aut-04-13
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Article

High Performance Liquid Chromatoqraphic Method for the Simultaneous Determination of Labetalol and Hydrochlorothiazide in Tablets and Spiked Human Plasma

by
M. Sultan
,
H. Abdine
,
N. Zoman
and
F. Belal
*
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh-11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2004, 72(2), 143-155; https://doi.org/10.3797/scipharm.aut-04-13
Submission received: 30 July 2003 / Revised: 23 January 2004 / Accepted: 23 January 2004 / Published: 7 June 2004
Versions Notes

Abstract

A reversed-phase HPLC method with spectrophotometric detection was developed for the simultaneous determination of labetalol (LBT) and hydrochloro-thiazide (HCD). The chromatographic separation was performed using a Microbondapak C18 column (4.6 i.d. x 250 nm) and paracetamol as internal standard. A mobile phase consisting of 0.05 M phosphate buffer/acetonitrile of pH 4 (7:3) at a flow rate of 0.7 ml/min was used. The detection was affected spectrophotornetrically at 302 nm. The working concentration range was 0.3–10 µg/ml with detection limits of 0.05 µg/ml for both drugs. The lower quantitation limit was 0.25 µg/ml in the two cases. The method was successfully applied to tablets, the % recoveries were 99.45 ± 0.68 for LBT and 99.79 ± 0.75 for HCD. The method was extended to the in-vitro determination in spiked human plasma. The % recoveries were 91.12 ± 0.33 for LBT and 91.37 ± 0.40 for HCD. The interday and intraday precision and accuracy were evaluated in plasma by calculating the % RSD (n=5) and the % error and were found to be in the ranges of 1.18–4.1% and 0.38–0.36% for both drugs, respectively.

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MDPI and ACS Style

Sultan, M.; Abdine, H.; Zoman, N.; Belal, F. High Performance Liquid Chromatoqraphic Method for the Simultaneous Determination of Labetalol and Hydrochlorothiazide in Tablets and Spiked Human Plasma. Sci. Pharm. 2004, 72, 143-155. https://doi.org/10.3797/scipharm.aut-04-13

AMA Style

Sultan M, Abdine H, Zoman N, Belal F. High Performance Liquid Chromatoqraphic Method for the Simultaneous Determination of Labetalol and Hydrochlorothiazide in Tablets and Spiked Human Plasma. Scientia Pharmaceutica. 2004; 72(2):143-155. https://doi.org/10.3797/scipharm.aut-04-13

Chicago/Turabian Style

Sultan, M., H. Abdine, N. Zoman, and F. Belal. 2004. "High Performance Liquid Chromatoqraphic Method for the Simultaneous Determination of Labetalol and Hydrochlorothiazide in Tablets and Spiked Human Plasma" Scientia Pharmaceutica 72, no. 2: 143-155. https://doi.org/10.3797/scipharm.aut-04-13

APA Style

Sultan, M., Abdine, H., Zoman, N., & Belal, F. (2004). High Performance Liquid Chromatoqraphic Method for the Simultaneous Determination of Labetalol and Hydrochlorothiazide in Tablets and Spiked Human Plasma. Scientia Pharmaceutica, 72(2), 143-155. https://doi.org/10.3797/scipharm.aut-04-13

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