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Volume 11
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10.3390/antibiotics11050577
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Article

Influence of Anti-Infective Periodontal Therapy on Subgingival Microbiota Evaluated by Chair-Side Test Compared to qPCR—A Clinical Follow-Up Study

by
Oliver Laugisch
1,
Thorsten M. Auschill
1,
Anne Tumbrink
1,2,
Anton Sculean
3 and
Nicole B. Arweiler
1,*
1
Department of Periodontology and Peri-Implant Diseases, Philipps-University, 35039 Marburg, Germany
2
Private Practice, 48324 Sendenhorst, Germany
3
Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland
*
Author to whom correspondence should be addressed.
Antibiotics 2022, 11(5), 577; https://doi.org/10.3390/antibiotics11050577
Submission received: 17 March 2022 / Revised: 14 April 2022 / Accepted: 21 April 2022 / Published: 26 April 2022
(This article belongs to the Special Issue Antibacterial Treatment in Periodontal and Endodontic Therapy)
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Abstract

A chair-side test (CST) for five periodontal pathogens (Aggregatibacter actinomycetemcomitans, A.a.; Porphyromonas gingivalis, P.g.; Prevotella intermedia, P.i.; Treponema denticola, T.d.; Tannerella forsythia, T.f.) was compared with qPCR in a previous clinical study on 100 periodontitis patients at first diagnosis (T0). Following non-surgical treatment alone (SRP) or in combination with systemic or local antibiotics, 74 patients (57.4 ± 13.5 years) were again tested at the same sites from 14 to 24 months after T0. Bacterial elimination (%; compared to T0) was determined for each single species and compared between both test systems. In all patients, all five pathogens could not be fully eliminated regardless of therapy or test method. Tested with CST, the mean elimination ranged from 90% for SRP + Amoxicillin/Metronidazole to 59.13% for SRP only. The corresponding qPCR values were 30% and 29.6%. Only A.a. was eradicated in 100% by SRP + Amoxicillin/Metronidazole tested by CST, and it was 80% when qPCR was the test method. CST agreed with qPCR in 98.7% in the detection of A.a., and 74.3%, 78.4%, 73.0%, and 48.7% for P.g., P.i., T.d., and T.f., respectively. Neither conventional treatment nor the additional use of antibiotics—even with the correct indication—could completely eradicate the tested pathogens or prevent pocket reinfection.
Keywords: periodontal therapy; supra- and subgingival debridement; anti-infective therapy; adjunctive antimicrobials; antibiotics; diagnosis; effectiveness; chair-side; point of care; evaluation periodontal therapy; supra- and subgingival debridement; anti-infective therapy; adjunctive antimicrobials; antibiotics; diagnosis; effectiveness; chair-side; point of care; evaluation

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MDPI and ACS Style

Laugisch, O.; Auschill, T.M.; Tumbrink, A.; Sculean, A.; Arweiler, N.B. Influence of Anti-Infective Periodontal Therapy on Subgingival Microbiota Evaluated by Chair-Side Test Compared to qPCR—A Clinical Follow-Up Study. Antibiotics 2022, 11, 577. https://doi.org/10.3390/antibiotics11050577

AMA Style

Laugisch O, Auschill TM, Tumbrink A, Sculean A, Arweiler NB. Influence of Anti-Infective Periodontal Therapy on Subgingival Microbiota Evaluated by Chair-Side Test Compared to qPCR—A Clinical Follow-Up Study. Antibiotics. 2022; 11(5):577. https://doi.org/10.3390/antibiotics11050577

Chicago/Turabian Style

Laugisch, Oliver, Thorsten M. Auschill, Anne Tumbrink, Anton Sculean, and Nicole B. Arweiler. 2022. "Influence of Anti-Infective Periodontal Therapy on Subgingival Microbiota Evaluated by Chair-Side Test Compared to qPCR—A Clinical Follow-Up Study" Antibiotics 11, no. 5: 577. https://doi.org/10.3390/antibiotics11050577

APA Style

Laugisch, O., Auschill, T. M., Tumbrink, A., Sculean, A., & Arweiler, N. B. (2022). Influence of Anti-Infective Periodontal Therapy on Subgingival Microbiota Evaluated by Chair-Side Test Compared to qPCR—A Clinical Follow-Up Study. Antibiotics, 11(5), 577. https://doi.org/10.3390/antibiotics11050577

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