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Biosensors
Volume 13
Issue 2
10.3390/bios13020195
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Communication

Complementary DNA Significantly Enhancing Signal Response and Sensitivity of a Molecular Beacon Probe to Aflatoxin B1

by
Chao Wang
1,
Kexiao Zhu
2,
Jie Yu
2 and
Pengfei Shi
1,*
1
College of Medicine, Linyi University, Linyi 276005, China
2
College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China
*
Author to whom correspondence should be addressed.
Biosensors 2023, 13(2), 195; https://doi.org/10.3390/bios13020195
Submission received: 22 December 2022 / Revised: 20 January 2023 / Accepted: 23 January 2023 / Published: 28 January 2023
(This article belongs to the Special Issue Biosensing and Diagnosis)
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Abstract

This paper reported an improved molecular beacon method for the rapid detection of aflatoxin B1 (AFB1), a natural mycotoxin with severe carcinogenicity. With the assistance of a complementary DNA (cDNA) chain, the molecular beacon which consists of a DNA aptamer flanked by FAM and BHQ1 displayed a larger fluorescent response to AFB1, contributing to the sensitive detection of AFB1. Upon optimization of some key experimental factors, rapid detection of AFB1 ranging from 1 nM to 3 μM, within 20 min, was realized by using this method. A limit of detection (LoD) of 1 nM was obtained, which was lower than the LoD (8 nM) obtained without cDNA assistance. This aptamer-based molecular beacon detection method showed advantages in easy operation, rapid analysis and larger signal response. Good specificity and anti-interference ability were demonstrated. This method showed potential in real-sample analysis.
Keywords: aflatoxin B1; aptamer; molecular beacon; complementary DNA; enhanced signal aflatoxin B1; aptamer; molecular beacon; complementary DNA; enhanced signal

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MDPI and ACS Style

Wang, C.; Zhu, K.; Yu, J.; Shi, P. Complementary DNA Significantly Enhancing Signal Response and Sensitivity of a Molecular Beacon Probe to Aflatoxin B1. Biosensors 2023, 13, 195. https://doi.org/10.3390/bios13020195

AMA Style

Wang C, Zhu K, Yu J, Shi P. Complementary DNA Significantly Enhancing Signal Response and Sensitivity of a Molecular Beacon Probe to Aflatoxin B1. Biosensors. 2023; 13(2):195. https://doi.org/10.3390/bios13020195

Chicago/Turabian Style

Wang, Chao, Kexiao Zhu, Jie Yu, and Pengfei Shi. 2023. "Complementary DNA Significantly Enhancing Signal Response and Sensitivity of a Molecular Beacon Probe to Aflatoxin B1" Biosensors 13, no. 2: 195. https://doi.org/10.3390/bios13020195

APA Style

Wang, C., Zhu, K., Yu, J., & Shi, P. (2023). Complementary DNA Significantly Enhancing Signal Response and Sensitivity of a Molecular Beacon Probe to Aflatoxin B1. Biosensors, 13(2), 195. https://doi.org/10.3390/bios13020195

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