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Article

Structural and Physicochemical Characterization of Chitosan Obtained by UAE and Its Effect on the Growth Inhibition of Pythium ultimum

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Center for Research and Assistance in Technology and Designo f the State of Jalisco, AC Centro de, CIATEJ Southeast Unit, Parque Científico Tecnológico de Yucatán, Km 5.5. Carretera Sierra Papacal—Chuburná Puerto, Mérida, Yucatán 97302, Mexico
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CONACYT—Center for Research and Assistance in Technology and Designo f the State of Jalisco, AC, Southeast Unit, Parque Científico Tecnológico de Yucatán, Km 5.5. Carretera Sierra Papacal—Chuburná Puerto, Mérida, Yucatán 97302, Mexico
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CIATEJ Zapopan Unit, Camino Arenero 1227, El Bajío del Arenal, 45019 Zapopan Jalisco, Mexico
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Ingénierie des Matériaux Polymères (IMP), CNRS UMR 5223, Université Claude Bernard Lyon 1, Univ Lyon, Villeurbanne F-69622, France
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Author to whom correspondence should be addressed.
Agriculture 2020, 10(10), 464; https://doi.org/10.3390/agriculture10100464
Received: 12 September 2020 / Revised: 3 October 2020 / Accepted: 5 October 2020 / Published: 9 October 2020
The objective of this work was the recovery of chitosan by ultrasound-assisted extraction (UAE) from white shrimp (Litopenaeus vannamei) chitin, and the physicochemical and structural characterization of the obtained biopolymer, as well as its antimicrobial effect on Pythium ultimum growth. A 23 factorial design was used to evaluate chitosan extraction conditions. Instrumental analysis techniques for chitosan characterization and radial growth inhibition, as an antifungal activity test, were performed. The ultrasonically extracted chitosan (UC) reached a yield of 86.96% with 100% solubility, a degree of deacetylation (DDA) >78%, molecular weight (MW) of 3.928 × 105 g mol−1, and a crystallinity index (Icr) of 87%, calculated through nuclear magnetic resonance (1H NMR) and Fourier transform infrared spectroscopy (FTIR), size exclusion chromatography (SEC), and X-ray diffraction (XRD), respectively. The inhibitory activity of the chitosan was evaluated against the oomycete Pythium ultimum, observing a 93% radial inhibition over 24 h. UAE proved to be an excellent alternative to the conventional deacetylation, reducing reaction time and obtaining a UC with higher MW and (Icr) than the commercial one, which could potentiate its applications. View Full-Text
Keywords: antimicrobial activity; chitosan; deacetylation; Litopenaeus vannamei; Pythium ultimum; ultrasound-assisted extraction antimicrobial activity; chitosan; deacetylation; Litopenaeus vannamei; Pythium ultimum; ultrasound-assisted extraction
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MDPI and ACS Style

Martín-López, H.; Pech-Cohuo, S.C.; Herrera-Pool, E.; Medina-Torres, N.; Cuevas-Bernardino, J.C.; Ayora-Talavera, T.; Espinosa-Andrews, H.; Ramos-Díaz, A.; Trombotto, S.; Pacheco, N. Structural and Physicochemical Characterization of Chitosan Obtained by UAE and Its Effect on the Growth Inhibition of Pythium ultimum. Agriculture 2020, 10, 464. https://doi.org/10.3390/agriculture10100464

AMA Style

Martín-López H, Pech-Cohuo SC, Herrera-Pool E, Medina-Torres N, Cuevas-Bernardino JC, Ayora-Talavera T, Espinosa-Andrews H, Ramos-Díaz A, Trombotto S, Pacheco N. Structural and Physicochemical Characterization of Chitosan Obtained by UAE and Its Effect on the Growth Inhibition of Pythium ultimum. Agriculture. 2020; 10(10):464. https://doi.org/10.3390/agriculture10100464

Chicago/Turabian Style

Martín-López, Héctor, Soledad C. Pech-Cohuo, Emanuel Herrera-Pool, Nelly Medina-Torres, Juan C. Cuevas-Bernardino, Teresa Ayora-Talavera, Hugo Espinosa-Andrews, Ana Ramos-Díaz, Stéphane Trombotto, and Neith Pacheco. 2020. "Structural and Physicochemical Characterization of Chitosan Obtained by UAE and Its Effect on the Growth Inhibition of Pythium ultimum" Agriculture 10, no. 10: 464. https://doi.org/10.3390/agriculture10100464

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