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Antibodies, Volume 13, Issue 3

September 2024 - 29 articles

Cover Story: Reducing the immunogenicity of animal-derived monoclonal antibodies (mAbs) for use in humans is critical to maximize therapeutic effectiveness and preclude potential adverse events. While traditional humanization methods have primarily focused on grafting antibody Complementarity-Determining Regions (CDRs) on homologous human antibody scaffolds, framework regions can also play essential roles in antigen binding. Here, we describe the humanization of the pan-HLA-DR mAb 44H10, a murine antibody displaying significant involvement of the framework region in antigen binding.  View this paper
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Articles (29)

  • Article
  • Open Access
3,336 Views
16 Pages

Novel Monoclonal Antibody Specific toward Amyloid-β Binds to a Unique Epitope within the N-Terminal Region

  • Giavanna Paterno,
  • Brenda D. Moore,
  • Brach M. Bell,
  • Kimberly-Marie M. Gorion,
  • Yong Ran,
  • Stefan Prokop,
  • Todd E. Golde and
  • Benoit I. Giasson

9 August 2024

Amyloid-β (Aβ) deposition throughout the neuroaxis is a classical hallmark of several neurodegenerative diseases, most notably Alzheimer’s disease (AD). Aβ peptides of varied length and diverse structural conformations are deposi...

  • Article
  • Open Access
1 Citations
2,799 Views
16 Pages

Identification of Conserved Linear Epitopes on Viral Protein 2 of Foot-and-Mouth Disease Virus Serotype O by Monoclonal Antibodies 6F4.D11.B6 and 8D6.B9.C3

  • Wantanee Tommeurd,
  • Kanyarat Thueng-in,
  • Sirin Theerawatanasirikul,
  • Nongnaput Tuyapala,
  • Sukontip Poonsuk,
  • Nantawan Petcharat,
  • Nattarat Thangthamniyom and
  • Porntippa Lekcharoensuk

7 August 2024

Foot-and-mouth disease (FMD) is a highly infectious disease of cloven-hoofed animals with a significant economic impact. Early diagnosis and effective prevention and control could reduce the spread of the disease which could possibly minimize economi...

  • Article
  • Open Access
3 Citations
2,932 Views
16 Pages

Enhanced N-Glycan Profiling of Therapeutic Monoclonal Antibodies through the Application of Upper-Hinge Middle-Up Level LC-HRMS Analysis

  • Natalia Mesonzhnik,
  • Anton Belushenko,
  • Polina Novikova,
  • Alexey Kukharenko and
  • Mikhail Afonin

6 August 2024

Therapeutic monoclonal antibodies (mAbs) are crucial in modern medicine due to their effectiveness in treating various diseases. However, the structural complexity of mAbs, particularly their glycosylation patterns, presents challenges for quality co...

  • Article
  • Open Access
1 Citations
2,657 Views
12 Pages

The Seraph 100® Microbind Affinity Blood Filter Does Not Alter Levels of Circulating or Mucosal Antibodies in Critical COVID-19 Patients

  • Tonia L. Conner,
  • Pooja Vir,
  • Eric D. Laing,
  • Ian J. Stewart,
  • Edward Mitre and
  • Kathleen P. Pratt

6 August 2024

PURIFY-OBS-1 is an observational study evaluating the safety and efficacy of Seraph 100® Microbind Affinity Blood Filter (Seraph 100) use for COVID-19 patients with respiratory failure admitted to the intensive care unit (ICU). The Seraph 100 is...

  • Article
  • Open Access
3,060 Views
24 Pages

1 August 2024

Critical blood shortages plague healthcare systems, particularly in lower-income and middle-income countries. This affects patients requiring regular transfusions and creates challenges during emergencies where universal blood is vital. To address th...

  • Communication
  • Open Access
1 Citations
1,762 Views
10 Pages

Knowledge of the Serological Response to the Third BNT162b2 Vaccination May Influence Compliance of Healthcare Workers to Booster Dose

  • Avi Magid,
  • Khetam Hussein,
  • Halima Dabaja-Younis,
  • Moran Szwarcwort-Cohen,
  • Ronit Almog,
  • Michal Mekel,
  • Avi Weissman,
  • Gila Hyams,
  • Vardit Gepstein and
  • Netanel A. Horowitz
  • + 4 authors

1 August 2024

Background: Previous studies showed that the fourth SARS-CoV-2 vaccine dose has a protective effect against infection, as well as against severe disease and death. This study aimed to examine whether knowledge of a high-level antibody after the third...

  • Article
  • Open Access
2,838 Views
16 Pages

1 August 2024

We aimed to develop a novel method for measuring the complement-binding ability of anti-blood type antibodies (ab-Abs), the flow cytometry method for the complement C1q test (FCM-C1q) for detecting antibody-mediated rejection (AMR) caused by ab-Abs i...

  • Review
  • Open Access
3,117 Views
15 Pages

The development of de novo donor-specific HLA antibodies (dnDSAs) following solid organ transplantation is considered a major risk factor for poor long-term allograft outcomes. The prediction of dnDSA development is a boon to transplant recipients, y...

  • Review
  • Open Access
2,800 Views
17 Pages

Application of Monoclonal Antibodies against Naturally Occurring Bioactive Ingredients

  • Shunsuke Fujii,
  • Takuhiro Uto,
  • Hiroaki Hayashi,
  • Waraporn Putalun,
  • Seiichi Sakamoto,
  • Hiroyuki Tanaka and
  • Yukihiro Shoyama

Monoclonal antibodies (Mabs) are widely used in a variety of fields, including protein identification, life sciences, medicine, and natural product chemistry. This review focuses on Mabs against naturally occurring active compounds. The preparation o...

  • Article
  • Open Access
16 Citations
6,704 Views
10 Pages

This study aimed to identify the 25 most prevalent adverse events (AEs) associated with FDA-approved immune checkpoint inhibitors (ICIs)—specifically, PD-1, PD-L1, CTLA-4, and LAG-3 inhibitors—using data from the FDA Adverse Events Report...

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Antibodies - ISSN 2073-4468