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Quantitative Evaluation of Ultrasound-Assisted Extraction of 1,3-β-glucans from Dictyophora indusiata Using an Improved Fluorometric Assay

Institute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Polymers 2019, 11(5), 864; https://doi.org/10.3390/polym11050864
Received: 13 March 2019 / Revised: 27 April 2019 / Accepted: 7 May 2019 / Published: 13 May 2019
(This article belongs to the Special Issue Food Polymers: Chemistry, Structure, Function and Application)
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Abstract

In the present study, an improved fluorometric assay based on aniline blue dye for the specific, accurate, and rapid quantification of 1,3-β-glucans in edible mushrooms was developed and fully validated. Furthermore, the improved method was successfully applied for the quantitative evaluation of water soluble 1,3-β-glucans extracted from Dictyophora indusiata by ultrasound-assisted extraction (UAE) with response surface methodology. Results showed that the improved method exhibited high specificity, accuracy, precision, repeatability, and stability, as well as a wide calibration range of 10–600 µg/mL (R2 > 99.9%). The maximum extraction yields of water soluble 1,3-β-glucans (1.20%) and total polysaccharides (5.41%) were achieved at the optimized extraction parameters as follows: ultrasound amplitude (56%), ultrasound extraction time (15 min), and ratio of liquid to raw material (22 mL/g). The results suggest that the improved fluorometric assay has great potential to be used as a routine method for the quantitative evaluation of 1,3-β-glucans in edible mushrooms and that the UAE method is effective for the extraction of 1,3-β-glucans from edible mushrooms. View Full-Text
Keywords: Dictyophora indusiata; 1,3-β-glucans; ultrasound-assisted extraction; fluorometric assay; aniline blue Dictyophora indusiata; 1,3-β-glucans; ultrasound-assisted extraction; fluorometric assay; aniline blue
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Fu, Y.; Lin, S.; Lu, M.; Wei, S.-Y.; Zhou, J.; Zhao, L.; Zhang, Q.; Lin, D.-R.; Liu, Y.-T.; Chen, H.; Qin, W.; Wu, D.-T. Quantitative Evaluation of Ultrasound-Assisted Extraction of 1,3-β-glucans from Dictyophora indusiata Using an Improved Fluorometric Assay. Polymers 2019, 11, 864.

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