Next Article in Journal
Looking for the “Dream Catalyst” for Hydrogen Peroxide Production from Hydrogen and Oxygen
Next Article in Special Issue
An Enzyme Cascade Synthesis of Vanillin
Previous Article in Journal
Optimization of Ammonia Oxidation Using Response Surface Methodology
Previous Article in Special Issue
Industrial Application of 2-Oxoglutarate-Dependent Oxygenases
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Catalysts 2019, 9(3), 250;

The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase

Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China
Authors to whom correspondence should be addressed.
Received: 18 January 2019 / Revised: 5 March 2019 / Accepted: 6 March 2019 / Published: 9 March 2019
(This article belongs to the Special Issue Biocatalysis for Industrial Applications)
Full-Text   |   PDF [4629 KB, uploaded 14 March 2019]   |  


Mannose-6-phosphate (M6P) is involved in many metabolic pathways in life, and it has important applications in the treatment of diseases. This study explored a cost-effective enzyme catalytic synthesis method of M6P, using polyphosphate-dependent mannose kinase from Arthrobacter species. This synthesis uses polyphosphate to replace expensive ATP, and it is greener and safer than chemical synthesis. This study investigated the effects of key factors such as metal ions, temperature, and substrate addition on this enzymatic reaction, and improved the conversion efficiency. We moreover take advantage of the response surface method to explore the best catalytic conditions synthetically. The conversion was 99.17% successful under the optimal reaction conditions. After a series of optimizations, we carried out a 200 mL scale-up experiment, which proved that the method has good prospects for industrial applications. View Full-Text
Keywords: mannose-6-phosphate; polyphosphate-dependent mannose kinase; polyphosphate mannose-6-phosphate; polyphosphate-dependent mannose kinase; polyphosphate

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Zhu, W.; Gao, M.; Chen, B.; Tan, T.; Cao, H.; Liu, L. The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase. Catalysts 2019, 9, 250.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Catalysts EISSN 2073-4344 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top