Investigation of Nicotianamine and 2′ Deoxymugineic Acid as Enhancers of Iron Bioavailability in Caco-2 Cells
Jesse T. Beasley 1,*
, Jonathan J. Hart 2, Elad Tako 2, Raymond P. Glahn 2 and Alexander A. T. Johnson 1
, Jonathan J. Hart 2, Elad Tako 2, Raymond P. Glahn 2 and Alexander A. T. Johnson 1
1
School of BioSciences, The University of Melbourne, Victoria 3010, Australia
2
Robert W. Holley Center for Agriculture and Health, USDA-ARS, Ithaca, NY 14853, USA
*
Author to whom correspondence should be addressed.
Nutrients 2019, 11(7), 1502; https://doi.org/10.3390/nu11071502
Received: 1 June 2019 / Revised: 25 June 2019 / Accepted: 28 June 2019 / Published: 30 June 2019
(This article belongs to the Special Issue Dietary Trace Minerals)
Abstract
Nicotianamine (NA) is a low-molecular weight metal chelator in plants with high affinity for ferrous iron (Fe2+) and other divalent metal cations. In graminaceous plant species, NA serves as the biosynthetic precursor to 2′ deoxymugineic acid (DMA), a root-secreted mugineic acid family phytosiderophore that chelates ferric iron (Fe3+) in the rhizosphere for subsequent uptake by the plant. Previous studies have flagged NA and/or DMA as enhancers of Fe bioavailability in cereal grain although the extent of this promotion has not been quantified. In this study, we utilized the Caco-2 cell system to compare NA and DMA to two known enhancers of Fe bioavailability—epicatechin (Epi) and ascorbic acid (AsA)—and found that both NA and DMA are stronger enhancers of Fe bioavailability than Epi, and NA is a stronger enhancer of Fe bioavailability than AsA. Furthermore, NA reversed Fe uptake inhibition by Myricetin (Myr) more than Epi, highlighting NA as an important target for biofortification strategies aimed at improving Fe bioavailability in staple plant foods. View Full-TextKeywords:
biofortification; iron deficiency anemia; iron absorption; ferritin; ascorbic acid; epicatechin
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Doi: 10.5281/zenodo.3236744
Link: https://zenodo.org/record/3236744#.XPIzidIzaUk
Description: Supplementary material for article submission to Nutrients 'Dietary Trace Minerals' special issue.
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Beasley, J.T.; Hart, J.J.; Tako, E.; Glahn, R.P.; Johnson, A.A.T. Investigation of Nicotianamine and 2′ Deoxymugineic Acid as Enhancers of Iron Bioavailability in Caco-2 Cells. Nutrients 2019, 11, 1502.
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