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Article

A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer

by
Han Cheng
1,
Jing Gao
1,
Ze-Wei An
1 and
Hua-Sun Huang
1,2,*
1
Key Laboratory of Rubber Biology, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou 571737, China
2
Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou 571737, China
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2010, 1(2), e14; https://doi.org/10.4081/pb.2010.e14
Submission received: 23 January 2010 / Revised: 26 July 2010 / Accepted: 27 July 2010 / Published: 11 August 2010
Versions Notes

Abstract

We have developed a rapid extraction method using low salt concentration buffer for the isolation of low-molecular-weight RNA from Arabidopsis tissues. The method was quick and efficient, and the small scale extraction process took no more than 1 hour, while yield and RNA quality were comparable with those of previously reported. The LMW RNA isolated using this method was high quality, abundant in small RNA and free of high molecular weight RNA. This method can be used to extract low-molecular-weight RNA for the purpose of small RNA cloning and detection, and library construction.
Keywords: low-molecular-weight RNA; low salt concentration buffer; Arabidopsis low-molecular-weight RNA; low salt concentration buffer; Arabidopsis

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MDPI and ACS Style

Cheng, H.; Gao, J.; An, Z.-W.; Huang, H.-S. A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer. Int. J. Plant Biol. 2010, 1, e14. https://doi.org/10.4081/pb.2010.e14

AMA Style

Cheng H, Gao J, An Z-W, Huang H-S. A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer. International Journal of Plant Biology. 2010; 1(2):e14. https://doi.org/10.4081/pb.2010.e14

Chicago/Turabian Style

Cheng, Han, Jing Gao, Ze-Wei An, and Hua-Sun Huang. 2010. "A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer" International Journal of Plant Biology 1, no. 2: e14. https://doi.org/10.4081/pb.2010.e14

APA Style

Cheng, H., Gao, J., An, Z. -W., & Huang, H. -S. (2010). A Rapid Method for Isolation of Low-Molecular-Weight Rna from Arabidopsis Using Low Salt Concentration Buffer. International Journal of Plant Biology, 1(2), e14. https://doi.org/10.4081/pb.2010.e14

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